PMID: 2116769Aug 15, 1990Paper

Characterization and primary sequence of a human hepatic microsomal estriol UDPglucuronosyltransferase

Archives of Biochemistry and Biophysics
B L CoffmanB Burchell

Abstract

A human liver microsomal UDP glucuronosyltransferase (UDPGT) that demonstrates reactivity with estriol (pI 7.4 UDPGT) has been purified to homogeneity and characterized further. No activity toward morphine, 4-hydroxybiphenyl, bilirubin, or tripelennamine was observed. The estriol UDPGT shows immunoreactivity with antibodies raised against rat hepatic microsomal 3 alpha- and 17 beta-hydroxysteroid UDPGTs but not with antibodies raised against rat hepatic microsomal p-nitrophenol UDPGT. The NH2-terminal sequence of the purified protein was determined and found to correspond to an identical sequence in the deduced amino acid sequence of a cDNA obtained from a human liver library in lambda gt11 (HLUG4). Sequence analysis revealed that HLUG4 is 2094 bp in length and encodes a protein of 523 amino acids which has a 16 amino acid leader sequence, followed by an untranslated 3' region of 525 bp. Three potential N-glycosylation sites were identified in the predicted sequence. The deduced amino acid sequence of estriol UDPGT showed 82% identity with the deduced amino acid sequence of another human hepatic cDNA (HLUG25), which has been expressed as a UDPGT capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid, strongly suggesti...Continue Reading

References

Aug 15, 1989·Archives of Biochemistry and Biophysics·M D Green, T R Tephly
Mar 1, 1987·The Biochemical Journal·M R JacksonB Burchell
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Citations

Jan 1, 1991·Pharmacology & Therapeutics·J O Miners, P I Mackenzie
Oct 21, 1999·The Journal of Steroid Biochemistry and Molecular Biology·W E GallA Radominska-Pandya
Oct 16, 1999·Glycobiology·D Kapitonov, R K Yu
Jan 1, 1995·The British Journal of Dermatology·F VecchiniB Shroot
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·K W Bock
Oct 25, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Liesbeth VandenputClaes Ohlsson
Sep 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T J HuangN R Chowdhury
Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P L JansenK W Bock
Jun 20, 2003·The Pharmacogenomics Journal·C Guillemette
May 15, 1997·Archives of Biochemistry and Biophysics·C J JinJ O Miners
Jul 24, 1998·Biochemical and Biophysical Research Communications·M BeaulieuA Bélanger
Apr 1, 1992·Biochemical Pharmacology·D Zakim, A J Dannenberg

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