Characterization and proteomic profiling of pancreatic cancer-derived serum exosomes

Journal of Cellular Biochemistry
Yu-J JiaoMingbing Xiao

Abstract

Pancreatic cancer (PC) is one of the most lethal cancers known worldwide, and its prognosis is poor in most patients. Exosomes are nanosized extracellular vesicles, which are released from various cell types. They are involved in cellular communication. The diagnosis and treatment of PC were improved substantially with exosomes. In this study, we isolated PC-derived exosomes and investigated their proteomic profile. Then, we conducted bioinformatic analysis on proteomic data. Differential ultracentrifugation was performed to isolate exosomes from human serum samples and four PC cell lines. Transmission electron microscopy and Western blot analysis were used to characterize the isolated exosomes. Liquid chromatography coupled with tandem mass spectrometry was conducted to identify the proteome of serum exosomes. Proteomic analysis demonstrated that all the serum exosomes were derived from three cohorts of human subjects; these serum exosomes contained a total of 655 proteins, out of which 315 proteins overlapped with ExoCarta database. Gene oncology and kyoto encyclopedia of genes and genomes analyses provided the functional annotation of the proteome. Interestingly, 18 or 14 proteins were upregulated and 11 or 14 proteins were ...Continue Reading

References

Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Jun 16, 2004·The Journal of Cell Biology·Alejandra TomasStephen E Moss
Aug 4, 2004·Cancer Research·Félix Fernández-MadridRobert L Karvonen
Jun 22, 2005·Pancreas·Xiaozhong Guo, Zhongmin Cui
Jun 28, 2005·Nucleic Acids Research·E QuevillonR Lopez
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Nov 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jin SongZhen Zhang
Dec 12, 2007·British Journal of Cancer·R DuncanG I Murray
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Mar 13, 2010·Molecular & Cellular Proteomics : MCP·Joanne L WeltonAled Clayton
May 12, 2011·Cellular and Molecular Life Sciences : CMLS·Bence GyörgyEdit I Buzás
Mar 15, 2012·Cancer Research·Melody A SwartzYves A DeClerck
Dec 20, 2012·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Kazuyoshi Motomura
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Marina ColomboClotilde Théry
Feb 17, 2015·Trends in Cell Biology·Emanuele Cocucci, Jacopo Meldolesi
Mar 18, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shuqing LiuMing-Zhong Sun
Apr 19, 2015·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Shoichi KinoshitaYoshiyuki Nakajima
Oct 1, 2015·Biochemical and Biophysical Research Communications·Min GeXiongzheng Mu
Oct 6, 2015·Journal of Molecular Biology·Shivakumar KeerthikumarSuresh Mathivanan
Nov 5, 2015·Advances in Experimental Medicine and Biology·Salvatore ScaràRoberto Scatena
Dec 29, 2015·Oncology Reports·Wiktoria M Suchorska, Michal S Lach
Feb 3, 2016·Lancet·Terumi KamisawaKyoichi Takaori
Aug 10, 2016·Biological Chemistry·Ursula Rescher, Thomas Grewal
Dec 14, 2016·Cancer Cell·Annette BeckerDavid Lyden
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Feb 9, 2017·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·H JinX Tan
Feb 28, 2017·International Journal of Cancer. Journal International Du Cancer·Bin ZhouHan-Xiang Zhan
Jul 19, 2017·Expert Review of Molecular Diagnostics·Celeste Caruso BavisottoClaudia Campanella
Sep 14, 2017·The Journal of Investigative Dermatology·Chang-Xian Wu, Zheng-Fei Liu
Oct 19, 2017·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J CastilloH Alvarez
Feb 10, 2018·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Emily A ArmstrongMary E Dillhoff

❮ Previous
Next ❯

Citations

Feb 5, 2019·Cancer Metastasis Reviews·Mariantonia LogozziStefano Fais
Apr 21, 2020·Current Pharmaceutical Design·Guifang ZhaoWenliang Li
Jun 17, 2020·Lab on a Chip·Guan HuangDayong Jin
Mar 8, 2019·International Journal of Molecular Sciences·Germana RappaAurelio Lorico
Oct 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing LiChenxi Yang
Mar 17, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Masatoshi ItoAi Kotani
Sep 2, 2021·Molecular and Cellular Biochemistry·Hejia GuoCuiping Li
Nov 9, 2021·Mass Spectrometry Reviews·Iqbal JalaludinJeongkwon Kim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Incidence & Mortality

Cancer has emerged as a global concern due to its increase in incidence and mortality. Efforts are underway to evaluate and develop action plans to reduce the global burden of cancer. Currently, lung cancer, breast cancer and prostate cancer are the leading causes of cancer mortality. Here is the latest research on cancer incidence and mortality.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Related Papers

Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
James D Perkins
Frontiers in Oncology
Meredith C Henderson, David O Azorsa
Cellular and Molecular Neurobiology
Mark Katakowski, Michael Chopp
© 2022 Meta ULC. All rights reserved