PMID: 1203320Jan 1, 1975Paper

Characterization and purification of messenger RNAs containing poly A in insect imaginal disks

Biochimie
P Tarroux

Abstract

The presence of a fragment of polyA resistant to both T1 and p ribonucleases in mRNAs extracted from wing imaginal disks of an insect, Pieris brassicae, is reported. Its length was approximatively 150 nucleotides. PolyU sepharose affinity chromatography was subsequently used for purification of these polyA(+)mRNA molecules. Analyses on sucrose gradients showed a good recovery of poly(+)molecules characterized by their size (20-100 S) and a polydisperse pattern. These mRNAlike species represent 2-3 per cent of the total radioactivity incorporated into RNA in 3 hours of labeling. Sequential extractions were carried out to provide cytoplasmic RNA rich fractions (4 degrees C) and nuclear rich fractions (45 degrees C). When assayed for the presence of polyA(+)RNA, molecules extracted by these two sequential methods were found to be very similar in their polyA content.

References

Mar 1, 1970·Zeitschrift Für Naturforschung. Teil B. Anorganische Chemie, Organische Chemie, Biochemie, Biophysik, Biologie·L F CongoteP Karlson
Dec 20, 1972·Nature: New Biology·B Daneholt
Feb 28, 1973·Nature: New Biology·D Sheiness, J E Darnell
Sep 28, 1973·Science·J E DarnellG R Molloy
Jun 1, 1973·FEBS Letters·J Reed, E Wintersberger
Jan 1, 1974·Annual Review of Biochemistry·G Brawerman
Jan 21, 1969·Biochimica Et Biophysica Acta·A J Howells, G R Wyatt
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J E DarnellR J Tushinski
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M EdmondsH Nakazato

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Citations

Dec 1, 1976·European Journal of Biochemistry·C Rodriguez-Pousada, D H Hayes
Feb 7, 1980·Journal of Theoretical Biology·R K Carlin

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