Characterization and quantification of ammonia-oxidizing bacteria in eutrophic coastal marine sediments using polyphasic molecular approaches and immunofluorescence staining

Environmental Microbiology
Hidetoshi UrakawaKunio Kohata

Abstract

Tokyo Bay, a eutrophic bay in Japan, receives nutrients from wastewater plants and other urban diffuse sources via river input. A transect was conducted along a line from the Arakawa River into Tokyo Bay to investigate the ecological relationship between the river outflow and the distribution, abundance and population structure of ammonia-oxidizing bacteria (AOB). Five surficial marine sediments were collected and analysed with polyphasic approaches. Heterogeneity and genetic diversity of beta-AOB populations were examined using restriction fragment length polymorphism (RFLP) analysis of 16S rRNA and amoA genes. A shift of the microbial community was detected in samples along the transect. Both 16S rRNA and amoA genes generated polymorphisms in the restriction profiles that were distinguishable at each sampling site. Two 16S rRNA gene libraries were constructed using the reverse transcription polymerase chain reaction (RT-PCR) method to determine the major ammonia oxidizers maintaining high cellular rRNA content. Two major groups were observed in the Nitrosomonas lineage; no Nitrosospira were detected. The effort to isolate novel AOB was successful; the isolate dominated in the gene libraries. For quantitative analysis, a real-...Continue Reading

References

May 1, 1988·Applied and Environmental Microbiology·D A StahlL Montgomery
Oct 1, 1981·Journal of Clinical Microbiology·A S Lampe
Nov 1, 1994·Journal of Bacteriology·A TeskeD A Stahl
Jul 15, 1994·FEMS Microbiology Letters·A E McCaigJ I Prosser
Aug 1, 1996·Applied and Environmental Microbiology·T A Hovanec, E F DeLong
Jun 1, 1996·Applied and Environmental Microbiology·B K MobarryD A Stahl
Dec 23, 2000·Nucleic Acids Research·B L MaidakJ M Tiedje
Sep 7, 2001·Annual Review of Microbiology·G A Kowalchuk, J R Stephen
Nov 28, 2001·Applied and Environmental Microbiology·P C BurrellT A Hovanec
Jan 5, 2002·International Journal of Systematic and Evolutionary Microbiology·A AakraI F Nes
May 8, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·M TorimuraR Kurane
Feb 5, 2003·Environmental Science & Technology·Gerda HarmsGary S Sayler
Mar 7, 2003·Applied and Environmental Microbiology·Thomas E Freitag, James I Prosser
Dec 9, 2003·Applied and Environmental Microbiology·Aurélie CébronJosette Garnier
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Jan 11, 2005·Applied and Environmental Microbiology·Sherry L DollhopfJoel E Kostka

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Citations

Dec 11, 2007·Applied and Environmental Microbiology·Hidetoshi UrakawaSatoshi Tsuneda
May 16, 2008·Environmental Microbiology·Hendrikus J Laanbroek, Arjen G C L Speksnijder
Oct 23, 2014·International Journal of Systematic and Evolutionary Microbiology·Hidetoshi UrakawaMartin G Klotz
Aug 27, 2010·FEMS Microbiology Reviews·Balbina NogalesRafael Bosch
Apr 11, 2017·Environmental Technology·Ji Hye JoJuntaek Lim
Jul 9, 2014·Applied and Environmental Microbiology·Hongchen JiangHailiang Dong
Nov 5, 2013·Applied and Environmental Microbiology·Yanling ZhengXunliang Cheng
Jun 29, 2014·The Journal of Microbiology·Maria MonteiroCatarina Magalhães

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