Characterization and regulation of AcrABR, a RND-type multidrug efflux system, in Agrobacterium tumefaciens C58

Microbiological Research
Puttamas NuonmingSkorn Mongkolsuk

Abstract

Agrobacterium tumefaciens AcrR is the transcriptional repressor of the acrABR operon. The AcrAB efflux pump confers resistance to various toxic compounds, including antibiotics [ciprofloxacin (CIP), nalidixic acid (NAL), novobiocin (NOV) and tetracycline (TET)], a detergent [sodium dodecyl sulfate (SDS)] and a biocide [triclosan (TRI)]. The sequence to which AcrR specifically binds in the acrA promoter region was determined by EMSA and DNase I footprinting. The AcrR-DNA interaction was abolished by adding NAL, SDS and TRI. Quantitative real time-PCR analysis showed that induction of the acrA transcript occurred when wild-type cells were exposed to NAL, SDS and TRI. Indole is a signaling molecule that increases the antibiotic resistance of bacteria, at least in part, through activation of efflux pumps. Expression of the A. tumefaciens acrA transcript was also inducible by indole in a dose-dependent manner. Indole induced protection against CIP, NAL and SDS but enhanced susceptibility to NOV and TRI. Additionally, the TET resistance of A. tumefaciens was not apparently modulated by indole. A. tumefaciens AcrAB played a dominant role and was required for tolerance to high levels of the toxic compounds. Understanding the regulation...Continue Reading

Citations

Jul 11, 2020·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Prabin ShresthaTit-Yee Wong
Aug 17, 2019·Frontiers in Plant Science·Thibault MeyerCéline Lavire
Jun 14, 2019·Oxidative Medicine and Cellular Longevity·Mohammad A Alfhili, Myon-Hee Lee
Sep 22, 2020·Journal of Environmental Science and Health. Part C, Toxicology and Carcinogenesis·Prabin ShresthaTit-Yee Wong
Jul 18, 2021·Microbiological Research·Jintana Duang-NkernSkorn Mongkolsuk
Aug 8, 2021·International Journal of Molecular Sciences·Fernando Sanz-GarcíaSara Hernando-Amado

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