Characterization and regulation of the latent transforming growth factor-beta complex secreted by vascular pericytes

Journal of Cellular Physiology
F MarraH E Abboud

Abstract

Transforming growth factor-beta (TGF-beta) stimulates the accumulation of extracellular matrix in renal and hepatic disease. Kidney glomerular mesangial cells (GMC) and liver fat-storing cells (FSC) produce latent of inactive TGF-beta. In this study, we characterized the latent TGF-beta complexes secreted by these cells. Human FSC produce a single latent TGF-beta complex, predominantly of the TGF-beta 1 isoform, whereas GMC secrete multiple complexes of latent TGF-beta, containing beta 1 and beta 2 isoforms. At least four forms were identified in GMC using ion exchange chromatography, including a peak not previously described in other cell types which eluted at 0.12 M NaCl, and predominantly of the beta 2 isoform. Both cell types secrete the latent TGF-beta 1 binding protein of 190 kDa, as part of a high molecular weight TGF-beta complex. Epidermal growth factor stimulates the secretion of latent TGF-beta and latent TGF-beta binding protein in both cell types. Secretion of latent TGF-beta in both cell types was found to be associated with secretion of decorin. This study shows that vascular pericytes from the kidney and the liver have distinctly different profiles of latent TGF-beta complexes, with GMC secreting a unique form o...Continue Reading

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Citations

Aug 17, 2000·Springer Seminars in Immunopathology·M Pinzani
Dec 28, 1999·Brain Research. Brain Research Reviews·W E Thomas
Mar 15, 2000·Brain Research Bulletin·H K RuckerW E Thomas
Aug 1, 2007·Comparative Hepatology·Olav A GressnerAxel M Gressner
Nov 2, 2004·Biological & Pharmaceutical Bulletin·Toshiyuki KajiThomas N Wight
Mar 13, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Olav A Gressner, Chunfang Gao
Nov 26, 2011·Oncology·Piotr K ZakrzewskiWanda M Krajewska
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Feb 7, 2002·Cardiovascular Drug Reviews·J Chamberlain
Jun 1, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·H YamabeK Okumura

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