PMID: 9524927Apr 3, 1998Paper

Characterization and representative structures of N-oligosaccharides bound to apolipoprotein H

Journal of Lipid Mediators and Cell Signalling
R GambinoM Cassader

Abstract

We studied the structure of N-linked carbohydrates bound to apolipoprotein H by a combination of two methods which make use of lectins. Digoxigenin-labelled lectins are used for the structural characterization of carbohydrate chains of glycoproteins. Concanavalin A lectin affinity chromatography was used to analyse apolipoprotein H according to the characteristics of its carbohydrate chain inner to sialic acid residues. Our results from digoxigenin-labelled lectins analysis showed that apolipoprotein H gave positive bands to SNA, DSA, GNA, PNA and AAA lectins. Apolipoprotein H gave a negative band when reacted with MAA lectin. When we applied apolipoprotein H onto the Concanavalin A lectin column no detectable amounts of protein were eluted with Concanavalin A buffer. After adding a buffer with low sugar concentration (10 mM glucoside) a large amount of apolipoprotein H was recovered. These molecules of apolipoprotein H weakly bound to the lectin. When a higher sugar concentration (500 mM mannoside) was added most of the sample applied was eluted. These molecules of apolipoprotein H firmly bound to the column having high affinity for the lectin. These results combined with those coming from the digoxigen-labeled lectins method ...Continue Reading

References

May 1, 1992·The Journal of Cell Biology·T Marquardt, A Helenius
Sep 1, 1992·The Journal of Clinical Investigation·A E GharaviK B Elkon
Jul 15, 1991·The Biochemical Journal·A SteinkassererA J Day
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H P McNeilS A Krilis
Jan 1, 1987·Annual Review of Biochemistry·S R Pfeffer, J E Rothman
Oct 29, 1986·Biochimica Et Biophysica Acta·J NimpfR F Zwaal
Jun 1, 1984·Archives of Biochemistry and Biophysics·J F CrowleyJ Lönngren
Jan 1, 1982·Annual Review of Biochemistry·G Ashwell, J Harford
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J LozierF W Putnam
Aug 14, 1980·Biochemical and Biophysical Research Communications·Y NakayaH B Brewer
Aug 1, 1994·Clinical Immunology and Immunopathology·T Koike
Aug 1, 1994·Lupus·D A Kandiah, S A Krilis
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Imperiali, K W Rickert
Sep 30, 1994·Atherosclerosis·M CassaderG Pagano
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J E HuntS A Krilis
May 1, 1997·Journal of Lipid Mediators and Cell Signalling·R GambinoM Cassader

❮ Previous
Next ❯

Citations

May 3, 2005·Journal of Chromatography. a·Ziping Yang, William S Hancock

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.