Characterization and Screening of Thermophilic Bacillus Strains for Developing Plant Growth Promoting Consortium From Hot Spring of Leh and Ladakh Region of India

Frontiers in Microbiology
Jay Prakash VermaVijai Singh

Abstract

In the present investigation, the main aim is to identify and characterize the potential drought tolerant plant growth promoting consortium for agricultural productivity. Three bacterial isolates were isolated from hot spring of Chumathang area of Leh district. Bacillus species (BHUJP-H1, BHUJP-H2, and BHUJP-H3) were done some biochemical tests including catalase, cellulase, amylase, indole-3-acetic acid, phosphate solubilisation, production of ammonia, siderophore, and hydrogen cyanide. Molecular characterization of isolates was done by 16S rDNA sequencing, e.g., Bacillus subtilis BHUJP-H1 (KU312403), Bacillus sp. BHUJP-H2 (KU312404) and B. licheniformis BHUJP-H3 (KU312405). The genetic diversity of the isolates was assessed by seven inter simple sequence repeat, all primer shows high polymorphism. The highest polymorphism efficiency and polymorphism information content showed by UBC-809 and UBC-836 which were 100% and 0.44 respectively, the lowest is by UBC-807 75% and 0.28 respectively. On an average 90.69% polymorphism efficiency and 0.40 polymorphism information contents obtained by used markers. The highest, 11.08 and the lowest, 4.50 effective multiplex ratios obtained for primer UBC-823 and UBC-807, on an average 7.99 e...Continue Reading

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Citations

Jan 10, 2020·Applied Microbiology and Biotechnology·Arpan MukherjeeAbd El-Latif Hesham
Jun 5, 2020·International Journal of Systematic and Evolutionary Microbiology·Mustafa Ozkan BaltaciAhmet Adiguzel
May 23, 2020·Current Microbiology·Trinetra MukherjeeSubhra Kanti Mukhopadhyay
Sep 26, 2020·PeerJ·Kiran KhandagaleAshutosh Rai
Aug 25, 2020·Microbial Pathogenesis·Maria Swiontek BrzezinskaUrszula Jankiewicz

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Methods Mentioned

BETA
PCR
electrophoresis
ISSR
genotyping

Software Mentioned

BLAST
Clustal W
MEGA
UPGMA
SPSS
NTSYSpc

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