Characterization and Standardization of Cultured Cardiac Fibroblasts for Ex Vivo Models of Heart Fibrosis and Heart Ischemia

Tissue Engineering. Part C, Methods
Yue ZhouPeipei Wang

Abstract

A full understanding of cardiac fibroblast (cFB) biology is essential to study the adverse cardiac remodeling and recovery of myocardium infarction. However, compared to cardiac myocytes, cFBs are less well characterized. Important questions, including the variability introduced by cell age (neonatal vs. adult), culture conditions (passage, plate coating, and culture medium), and responses to stimuli (e.g., hypoxia and drug treatments), have not been well addressed and standardization of techniques is lacking. This variability invites inconsistency and the confounding of study conclusions. Thus, we here focus on characterizing cell responses and standardizing procedures for cFB isolation and culture conditions to provide reliable platforms to address important questions about cFB proliferation, activation, collagen matrix formation, and responses to relevant stimuli. Thirty litters of 1-3-day pups and 30 female (240-330 g) Sprague-Dawley rats were used to isolate neonatal and adult cFBs. We detail and validate procedures to isolate cFBs for the use of culture or direct analysis. We characterize the differences between neonatal and adult cFBs, define the changes of cFBs during serial passage, and identify the response of cFBs to...Continue Reading

References

Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R T FranceschiY Cui
Sep 13, 2001·Molecular Biology of the Cell·B HinzC Chaponnier
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·James D Stockand, J Gary Meszaros
May 14, 2003·Trends in Cell Biology·Frederick Grinnell
Jun 9, 2004·The Journal of Biological Chemistry·Maritza MayorgaDaniel Sanchis
Jul 16, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Edie C GoldsmithThomas K Borg
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
May 1, 2007·Nature Medicine·Alessandra CarèGianluigi Condorelli
Jul 3, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Indroneal BanerjeeTroy A Baudino
Dec 4, 2008·Journal of Tissue Engineering and Regenerative Medicine·E HadjipanayiR A Brown
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky
Dec 17, 2009·Fibrogenesis & Tissue Repair·Clarice Zc Chen, Michael Raghunath
Apr 30, 2010·Journal of Cell Science·Lysianne Follonier CastellaBoris Hinz
May 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jon-Jon SantiagoIan M C Dixon
Feb 18, 2011·Journal of Cardiovascular Pharmacology·Stephan Rohr
Apr 28, 2012·Circulation Research·Tilanthi M JayawardenaVictor J Dzau
Mar 22, 2014·Circulation Research·Milena B FurtadoNadia A Rosenthal
Apr 20, 2014·The Journal of Clinical Investigation·Claudia BangThomas Thum
Jun 18, 2014·The Journal of Clinical Investigation·Thomas Moore-MorrisSylvia M Evans
Oct 7, 2014·Soft Matter·M Yakut AliM Taher A Saif
Jan 7, 2015·Cardiovascular Research·James E CartledgeCesare M Terracciano
Dec 5, 2015·Circulation Research·Alexander R PintoMichelle D Tallquist
Oct 26, 2016·Differentiation; Research in Biological Diversity·Jessica M SwongerMichelle D Tallquist

❮ Previous
Next ❯

Citations

Mar 11, 2020·Frontiers in Endocrinology·Jiri Pavlacky, Jan Polak
Oct 30, 2018·Journal of the American Heart Association·Josefin SoppertChristian Stoppe
Jun 7, 2019·Journal of Cardiovascular Development and Disease·Kartika R PertiwiEmily Lisa Chilton
Oct 17, 2020·Veterinary World·Kittipong Tachampa, Tuempong Wongtawan

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

gentleMACS
GraphPad prism

Related Concepts

Related Feeds

Cardiac Remodeling

Cardiac remodeling in response to a myocardial infarction is characterized by progressive ventricular dilatation, cardiac hypertrophy, fibrosis, and deterioration of cardiac performance. Discover the latest research on Cardiac Remodeling here.