Characterization and Structural Analysis of a Novel exo-Type Enzyme Acting on β-1,2-Glucooligosaccharides from Parabacteroides distasonis

Biochemistry
Hisaka ShimizuHayao Taguchi

Abstract

β-1,2-Glucan is a polysaccharide produced mainly by some Gram-negative bacteria as a symbiosis and infectious factor. We recently identified endo-β-1,2-glucanase from Chitinophaga pinensis ( CpSGL) as an enzyme comprising a new family. Here, we report the characteristics and crystal structure of a CpSGL homologue from Parabacteroides distasonis, an intestinal bacterium (BDI_3064 protein), which exhibits distinctive properties of known β-1,2-glucan-degrading enzymes. BDI_3064 hydrolyzed linear β-1,2-glucan and β-1,2-glucooligosaccharides with degrees of polymerization (DPs) of ≥4 to produce sophorose specifically but did not hydrolyze cyclic β-1,2-glucan. This result indicates that BDI_3064 is a new exo-type enzyme. BDI_3064 also produced sophorose from β-1,2-glucooligosaccharide analogues that have a modified reducing end, indicating that BDI_3064 acts on its substrates from the nonreducing end. The crystal structure showed that BDI_3064 possesses additional N-terminal domains 1 and 2, unlike CpSGL. Superimposition of BDI_3064 and CpSGL complexed with ligands showed that R93 in domain 1 overlapped subsite -3 in CpSGL. Docking analysis involving a β-1,2-glucooligosaccharide with DP4 showed that R93 completely blocks the nonreduc...Continue Reading

References

Jul 1, 1990·Applied and Environmental Microbiology·M W BreedveldA J Zehnder
Mar 1, 1989·Journal of Bacteriology·G A CangelosiE W Nester
May 1, 1972·Analytical Biochemistry·M Lever
Nov 1, 1994·Journal of Bacteriology·P TalagaJ P Bohin
Jun 1, 1994·Microbiological Reviews·M W Breedveld, K J Miller
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
Nov 5, 1997·Current Opinion in Structural Biology·B Henrissat, G Davies
Apr 26, 2000·FEMS Microbiology Letters·J P Bohin
Mar 9, 2002·Journal of Molecular Biology·Diego M A GuérinPedro M Alzari
Jun 26, 2003·Nucleic Acids Research·Takeshi Kawabata
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Agnieszka S JunckerAnders Krogh
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Oct 19, 2004·Journal of Bacteriology·Andrés E CiocchiniRodolfo A Ugalde
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
May 10, 2005·Nature Immunology·Beatriz Arellano-ReynosoJean-Pierre Gorvel
Nov 30, 2005·The Journal of Biological Chemistry·Shabir NajmudinCarlos M G A Fontes
Aug 25, 2006·Acta Crystallographica. Section D, Biological Crystallography·Kevin Cowtan
Nov 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jason S McLellanDaniel J Leahy
Jun 21, 2007·PLoS Biology·Jian XuJeffrey I Gordon
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Oct 2, 2007·The Journal of Biological Chemistry·Yu KitagoIsao Tanaka
Oct 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrés E CiocchiniRodolfo A Ugalde
Dec 7, 2007·Molecular Plant-microbe Interactions : MPMI·Alejandra L D'AntuonoViviana C Lepek
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael A MahowaldJeffrey I Gordon
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Alexei Vagin, Alexei Teplyakov
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Jan 8, 2011·Archiv der Pharmazie·Goutam BrahmachariArun K Brahmachari
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Aug 15, 2012·Journal of Cheminformatics·Marcus D HanwellGeoffrey R Hutchison
May 15, 2013·Nucleic Acids Research·Hamish McWilliamRodrigo Lopez
Nov 26, 2013·Nucleic Acids Research·Vincent LombardBernard Henrissat
Mar 22, 2014·PloS One·Masahiro NakajimaMotomitsu Kitaoka
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Oct 31, 2014·Bioinformatics·Nicolas TerraponBernard Henrissat
Jan 6, 2016·Applied and Environmental Microbiology·Keith MewisBernard Henrissat
May 1, 2016·Nucleic Acids Research·Liisa Holm, Laura M Laakso

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