Characterization and structure identification of an antimicrobial peptide, hominicin, produced by Staphylococcus hominis MBBL 2-9

Biochemical and Biophysical Research Communications
Pyoung Il KimByung-Gee Kim

Abstract

Hominicin, antimicrobial peptide displaying potent activity against Staphylococcus aureus ATCC 25923, methicillin-resistant S. aureus (MRSA) ATCC 11435 and vancomycin-intermediate S. aureus (VISA) CCARM 3501, was purified by chloroform extraction, ion-exchange column chromatography and reverse-phase HPLC from culture supernatant of Staphylococcushominis MBBL 2-9. Hominicin exhibited heat stability up to 121 degrees C for 15min and activity under both acidic and basic conditions (from pH 2.0 to 10.0). Hominicin was cleaved into two fragments after treatment with proteinase K, resulting in the loss of its antibacterial activity, while it was resistant to trypsin, alpha-chymotrypsin, pepsin and lipase. The molecular mass of hominicin determined by mass spectrometry was 2038.4Da. LC-mass spectrometry and NMR spectroscopy analyses of the two fragments revealed the sequence of hominicin as DmIle-Dhb-Pro-Ala-Dhb-Pro-Phe-Dhb-Pro-Ala-Ile-Thr-Glu-Ile-Dhb-Ala-Ala-Val-Ile-Ala-Dmp, which had no similarity with other antimicrobial peptides previously reported. The present study is the first report of this novel antimicrobial peptide, which has uncommon amino acid residues like the ones in Class I group and shows potent activity against clini...Continue Reading

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Citations

Oct 15, 2013·International Journal of Medical Microbiology : IJMM·Friedrich GötzMartin Schlag
Dec 9, 2014·Jundishapur Journal of Microbiology·Gholam Hossein EbrahimipourAtusa Aliahmadi
Oct 31, 2012·Cellular and Molecular Life Sciences : CMLS·Riadh HammamiIsmail Fliss
Jun 5, 2015·Archives of Microbiology·T SuwandechaK Pangsomboon
May 29, 2016·International Journal of Food Microbiology·María Sánchez MainarFrédéric Leroy
Jun 8, 2017·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Anongtip SaTeerapol Srichana
May 13, 2020·FEMS Microbiology Ecology·Julie N O'SullivanR Paul Ross
Nov 1, 2020·Applied Microbiology and Biotechnology·Maria do Carmo de Freire BastosMarcus Lívio Varella Coelho
Feb 10, 2021·Pathogens·Angela FrançaLuís D R Melo
May 22, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·James FlynnSarah P Hudson
Jul 15, 2021·International Journal of Microbiology·Ellen TwomeyMáire Begley
Aug 8, 2021·International Journal of Molecular Sciences·Isabella Hernández-Aristizábal, Iván Darío Ocampo-Ibáñez

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