PMID: 9445382Mar 7, 1998Paper

Characterization and sugar-binding properties of arcelin-1, an insecticidal lectin-like protein isolated from kidney bean (Phaseolus vulgaris L. cv. RAZ-2) seeds

The Biochemical Journal
C FabreP Rougé

Abstract

Arcelin-1 is a lectin-like protein found in the seeds of wild varieties of the kidney bean (Phaseolus vulgaris). This protein displays insecticidal properties, but the mechanism of action is as yet unknown. In the present study we investigated the biochemical and biophysical properties of arcelin-1 from Phaseolus vulgaris cv. RAZ-2. Native arcelin-1 is a dimeric glycoprotein of 60 kDa, built from the non-covalent association of two identical monomers. This dimer resists dissociation by chaotropic agents and is highly resistant to proteolytic enzymes. Each subunit contains 10% (w/w) neutral sugars which belong to the high-mannose and complex-type glycans attached to three glycosylation sites. No interaction of the protein with simple sugars could be detected, but arcelin-1 displays an intrinsic specificity in binding complex glycans. Arcelin-1 therefore differs from the closely related phytohaemagglutinin lectins and alpha-amylase inhibitor in several respects: oligomerization states, sugar-binding affinities and the type and number of glycan chains. These features may be related to the toxicity of arcelin-1.

Citations

Oct 3, 2013·Plant Signaling & Behavior·A G Ingale, A U Hivrale
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Yoshiaki KouzumaMasami Yonekura
Nov 22, 2017·The Plant Journal : for Cell and Molecular Biology·Elia StahlPhilippe Reymond
Apr 4, 2009·Journal of Biomedicine & Biotechnology·Arishya SharmaPeng Lin
Oct 16, 2004·The Journal of Peptide Research : Official Journal of the American Peptide Society·P H K Ngai, T B Ng
Jan 23, 1999·European Journal of Biochemistry·E J Van DammeW J Peumans
May 28, 1998·The Journal of Biological Chemistry·L MoureyJ P Samama
Aug 10, 2000·European Journal of Biochemistry·E J Van DammeW J Peumans
Nov 3, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Isabelle ZauggFrancesca Sparvoli
Aug 15, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Patrick H K Ngai, T B Ng
Jun 10, 2008·Journal of Agricultural and Food Chemistry·Akiko Shimizu-IbukaKeiko Abe
Nov 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Célia R Carlini, Maria Fátima Grossi-de-Sá
Mar 14, 2007·Journal of Agricultural and Food Chemistry·Ana Paula de A BoletiMaria Lígia R Macedo
Apr 23, 2003·Biochemical and Biophysical Research Communications·Pierre RougéEls J M Van Damme
Oct 12, 2000·Plant Physiology·M P SalesJ Xavier-Filho
Aug 10, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Maria Lígia R MacedoJosé Roberto P Parra
Feb 12, 2008·Journal of Agricultural and Food Chemistry·Sundaram JanarthananBrenda Oppert
Jan 29, 2005·Journal of Agricultural and Food Chemistry·Maria Lígia Rodrigues MacedoLuana Cassandra B B Coelho
Oct 1, 2011·Biotechnology Advances·Parvaiz AhmadF Al-Qurainy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.