Characterization and survival of environmental Escherichia coli O26 isolates in ground beef and environmental samples

Journal of Food Science
Christine E PalmerLuxin Wang

Abstract

In addition to Escherichia coli O157:H7, shiga toxin-producing E. coli (STEC) O26 was added to the zero-tolerance adulterant list together with other 5 non-O157 STEC serogroups in 2012. Four farm O26 isolates were used in this study; they were obtained from a on-farm survey study conducted in Alabama. The presence of 3 major pathogenic genes (stx1, stx2, and eaeA) was determined through multiplex polymerase chain reaction (PCR). Two major pathogenic gene profiles were observed: 3 of the farm isolates contain only the eaeA gene whereas 1 farm isolate has both the eaeA and the stx1 genes. No significant difference was seen among the 4 farm isolates in the antibiotic resistance tests. To test their survival in ground beef and environmental samples, 2 inoculums were prepared and inoculated at various concentrations into samples of ground beef, bovine feces, bedding materials, and trough water. One inoculum was made of 3 farm isolates containing only the eaeA gene and another inoculum contained the isolate with both the eaeA and stx1 genes. Inoculated beef samples were stored at 4 °C for 10 d and the inoculated environmental samples were stored at ambient temperature for 30 d. Results showed that virulence gene profiles do not have ...Continue Reading

References

Jul 1, 1996·Applied and Environmental Microbiology·G WangM P Doyle
Nov 1, 1996·The Journal of Infectious Diseases·P I Tarr, M A Neill
Apr 12, 2001·Letters in Applied Microbiology·M A EhrmannR F Vogel
Aug 26, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L Beutin
Dec 23, 2006·Journal of Applied Microbiology·B FremauxC Vernozy-Rozand
Jun 28, 2007·Letters in Applied Microbiology·B FremauxC Vernozy-Rozand
Sep 11, 2010·Journal of Food Protection·Emily C MathusaLloyd Hontz
Aug 10, 2011·PloS One·Jincai MaChing-Hong Yang
Aug 13, 2011·Frontiers in Microbiology·Brian K CoombesChelsey D Goodman
Oct 18, 2011·Applied and Environmental Microbiology·Aine MonaghanDeclan J Bolton
Apr 30, 2013·Virulence·Analía Inés Etcheverría, Nora Lía Padola

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.