Abstract
In addition to Escherichia coli O157:H7, shiga toxin-producing E. coli (STEC) O26 was added to the zero-tolerance adulterant list together with other 5 non-O157 STEC serogroups in 2012. Four farm O26 isolates were used in this study; they were obtained from a on-farm survey study conducted in Alabama. The presence of 3 major pathogenic genes (stx1, stx2, and eaeA) was determined through multiplex polymerase chain reaction (PCR). Two major pathogenic gene profiles were observed: 3 of the farm isolates contain only the eaeA gene whereas 1 farm isolate has both the eaeA and the stx1 genes. No significant difference was seen among the 4 farm isolates in the antibiotic resistance tests. To test their survival in ground beef and environmental samples, 2 inoculums were prepared and inoculated at various concentrations into samples of ground beef, bovine feces, bedding materials, and trough water. One inoculum was made of 3 farm isolates containing only the eaeA gene and another inoculum contained the isolate with both the eaeA and stx1 genes. Inoculated beef samples were stored at 4 °C for 10 d and the inoculated environmental samples were stored at ambient temperature for 30 d. Results showed that virulence gene profiles do not have ...Continue Reading
References
Jul 1, 1996·Applied and Environmental Microbiology·G WangM P Doyle
Nov 1, 1996·The Journal of Infectious Diseases·P I Tarr, M A Neill
Feb 18, 1998·Journal of Clinical Microbiology·A W Paton, J C Paton
Feb 9, 2000·Veterinary Microbiology·R Cobbold, P Desmarchelier
Jun 2, 2000·Journal of Clinical Microbiology·W L ZhangH Karch
Apr 12, 2001·Letters in Applied Microbiology·M A EhrmannR F Vogel
Sep 18, 2002·Journal of Food Protection·Vijay K Sharma
Mar 9, 2004·Journal of Clinical Microbiology·Lothar BeutinKerstin Gleier
Apr 14, 2004·Journal of Applied Microbiology·M VialetteM Lange
Jul 14, 2006·Veterinary Microbiology·B FremauxC Vernozy Rozand
Aug 26, 2006·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L Beutin
Dec 23, 2006·Journal of Applied Microbiology·B FremauxC Vernozy-Rozand
Jun 28, 2007·Letters in Applied Microbiology·B FremauxC Vernozy-Rozand
Sep 11, 2010·Journal of Food Protection·Emily C MathusaLloyd Hontz
Nov 16, 2010·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Po-Ren HsuehLee-Jene Teng
May 10, 2011·Vaccine·Vijay K SharmaThomas A Casey
Aug 10, 2011·PloS One·Jincai MaChing-Hong Yang
Aug 13, 2011·Frontiers in Microbiology·Brian K CoombesChelsey D Goodman
Oct 18, 2011·Applied and Environmental Microbiology·Aine MonaghanDeclan J Bolton
Apr 30, 2013·Virulence·Analía Inés Etcheverría, Nora Lía Padola