Characterization and targeted disruption of a glycosyltransferase gene in the tylosin producer, Streptomyces fradiae

Gene
V T Wilson, Eric Cundliffe

Abstract

An open reading frame, designated tylN, has been identified by sequence analysis at one end of the tylosin biosynthetic gene cluster of Streptomyces fradiae, alongside a cluster of genes encoding the biosynthesis of dTDP-deoxyallose. This 6-deoxyhexose sugar is converted to mycinose, via bis O-methylation, following attachment to the polyketide lactone during tylosin biosynthesis. The deduced product of tylN is similar to several glycosyltransferases, authentic and putative, and displays a consensus sequence motif that appears to be characteristic of a sub-group of such enzymes. Specific disruption of tylN within the S. fradiae genome resulted in the production of demycinosyl-tylosin, whereas other glycosyltransferase activities involved in tylosin biosynthesis were not affected. Evidently, tylN encodes deoxyallosyl transferase.

References

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Citations

Jan 14, 2010·Molecules and Cells·Binod Babu PageniJae Kyung Sohng
May 3, 2002·Molecular Microbiology·Neil BateEric Cundliffe
Nov 24, 2004·Molecular Microbiology·George StratigopoulosEric Cundliffe
Sep 1, 2006·Molecular Microbiology·Neil BateEric Cundliffe
Apr 3, 2001·Microbiology·A R ButlerE Cundliffe
Jun 11, 2002·Microbiology·Magdalena KotowskaKatarzyna Kuczek
May 14, 1999·Chemistry & Biology·A R ButlerE Cundliffe
Jul 28, 2016·Journal of Natural Products·Eunji KimYeo Joon Yoon
Jun 29, 2002·Nature Biotechnology·Andrew R ButlerEric Cundliffe
Feb 14, 2002·Chemistry & Biology·George Stratigopoulos, Eric Cundliffe
Jul 25, 2003·Journal of Medicinal Chemistry·Christopher WalshHeather C Losey

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