Characterization and transformation potential of "Synthetic" astrocytes differentiated from murine embryonic stem cells

Glia
Deepak KamnasaranAbhijit Guha

Abstract

Our objective was to determine if murine embryonic stem (ES) cells, which are readily available from repositories, could be developed as a model of gliomagenesis, recognizing the difficulty in obtaining and transforming somatic astrocytes. Using a stringently controlled sequential differentiation procedure on wild type (wt) and p53+/- ES cells, we established GFAP+A2B5-synthetic astrocytes with high efficiency (>90%). The synthetic astrocytes stably express several differentiated astrocyte associated structural proteins and biochemical markers, but lacked expression of differentiated neurons and oligodendrocytes. However, in contrast to somatic differentiated astrocytes, the synthetic astrocytes expressed stem cell markers, with a transcriptome profile similar to astrocytes differentiated from neural stem cells (NSC) and somatic astrocyte cultures established from E13.5-Cortex and P4-hippocampus. In addition, the synthetic astrocytes demonstrated plasticity, with ability to dedifferentiate into neuronal and oligodendrocyte lineages. Intracranial injection of postnatal differentiated somatic astrocytes or synthetic astrocytes of either wt or p53+/- background did not grow tumors, unlike corresponding ES cells that develop terato...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S J FieldM E Greenberg
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·J A ThomsonJ P Hearn
Aug 1, 1996·Biology of Reproduction·J A ThomsonJ P Hearn
Apr 4, 1997·Brain Research·K J SeidmanJ A Weyhenmeyer
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M S RaoM Mayer-Pröschel
Aug 11, 1998·Nature Genetics·A Di CristofanoP P Pandolfi
Nov 6, 1998·Science·J A ThomsonJ M Jones
Sep 16, 1999·FEBS Letters·K NakashimaT Taga
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Oct 23, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H SuemoriN Nakatsuji
Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Dec 4, 2001·Nature Biotechnology·B E ReubinoffT Ben-Hur
Dec 12, 2001·Science·J M Penninger, J Woodgett
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lars M BjorklundOle Isacson
Oct 31, 2002·Molecular Endocrinology·Hoi Sang UFarid Saljooque
Oct 30, 2003·Nature Neuroscience·Fiona Doetsch
May 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Robert M BachooRonald A DePinho
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Aug 30, 2005·The American Journal of Pathology·Patrick ShannonAbhijit Guha
Dec 1, 2006·Expert Review of Anticancer Therapy·Jeremy N Rich
Apr 28, 2007·Proceedings of the National Academy of Sciences of the United States of America·Deepak KamnasaranAbhijit Guha

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Citations

Oct 8, 2015·Trends in Molecular Medicine·Chen ChenPeng Jiang

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