Characterization and virulence of hemolysin III from Vibrio vulnificus

Current Microbiology
Yu-Chung ChenChii-Ling Jeang

Abstract

Vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. A gene (hlyIII) encoding a hemolysin was cloned and sequenced from V. vulnificus. Nucleotide sequence analysis predicted an open reading frame of 642 bp encoding a 214 amino acid polypeptide that showed 48% sequence identity to the hemolysin III of Bacillus cereus. When HlyIII of V. vulnificus was expressed in Escherichia coli, crude extracts exhibited hemolytic activity similar to that of hemolysin III from Bacillus cereus. A hlyIII isogenic mutant was constructed via insertional inactivation and showed an attenuated virulence compared with the wild-type strain when this mutant was administered intraperitoneally in mice.

Citations

Oct 20, 2011·Journal of Molecular Evolution·Nancy Y VillaThomas J Lyons
Aug 24, 2011·Canadian Journal of Microbiology·Ariadnna CruzIrma Rosas
Jul 8, 2011·Biology Direct·Jimin PeiNick V Grishin
Apr 20, 2005·Journal of Applied Microbiology·X-H Zhang, B Austin
Oct 11, 2017·International Journal of Environmental Research and Public Health·Charles A Osunla, Anthony I Okoh
Jun 6, 2018·Frontiers in Microbiology·Bart A EijkelkampChristopher A McDevitt
Feb 23, 2020·Journal of Food Protection·Julio Parra-FloresOndrej Holy
Sep 10, 2019·Frontiers in Microbiology·Brady L SpencerKelly S Doran
Aug 29, 2017·Molecular Oral Microbiology·Hidenobu SenpukuNaoki Narisawa

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