Characterization, expression, and function analysis of gonad-inhibiting hormone in Oriental River prawn, Macrobrachium nipponense and its induced expression by temperature

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
Hui QiaoYongsheng Gong

Abstract

Gonad-inhibiting hormone (GIH) is a member of crustacean hyperglycemic hormone family and plays a major role in regulating reproduction in crustaceans. In this study, a full-length cDNA of GIH of Oriental River prawn, Macrobrachium nipponense (Mn-GIH) was cloned from the eyestalk. A 1350 bp full-length Mn-GIH cDNA harbored 336 bp of an open reading frame encoding signal peptide of 112 amino acid residues. Sequence analysis revealed that the overall cDNA sequence and specific functional sites of Mn-GIH were highly conserved with those in other crustacean species. Expression analysis by quantitative real-time PCR demonstrated its tissue-specific, larval developmental stage-specific, and ovary developmental stage-specific expression pattern, respectively. The RNAi by GIH-ds-RNA in vivo injection was effective in this study and resulted a 50% (day 1), 83% (day 5) and 63% (day 9) down-regulation compared to control. The obvious changes of gonad somatic index (GSI) rate also provided strong evidence to the inhibition effects of GIH on ovary maturation and spawning. Four temperature gradients (12 °C ± 1 °C, 17 °C ± 1 °C, 22 °C ± 1 °C, 27 °C ± 1 °C) were set to imitate the temperature in breeding and non-breeding seasons. The observed ...Continue Reading

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Citations

Feb 18, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Shengming SunWenyi Zhang
Aug 4, 2018·International Journal of Molecular Sciences·Yuning HuYan Wu
Apr 11, 2019·International Journal of Molecular Sciences·Yabing WangYan Wu
Nov 14, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hui QiaoHongtuo Fu
Feb 23, 2020·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Yuning ZhangYan Wu
Aug 30, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Lei XuYuning Hu

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