Characterization, expression and subcellular distribution of a novel MFP1 (matrix attachment region-binding filament-like protein 1) in onion.

Protoplasma
Rafael SamaniegoS Moreno Díaz de la Espina

Abstract

MFP1 (matrix attachment region-binding filament-like protein 1) is a conserved nuclear and chloroplast DNA-binding protein encoded by a nuclear gene, well characterized in dicot species. In monocots, only a 90 kDa MFP1-related protein had been characterized in the nucleus and nuclear matrix of Allium cepa proliferating cells. We report here a novel MFP1-related nuclear protein of 80 kDa in A. cepa roots, with M(r) and pI values similar to those of MFP1 proteins in dicot species, and which also displays a dual location, in the nucleus and chloroplasts of leaf cells. However, this novel protein is not a nuclear matrix component. It shows a spotted intranuclear distribution in small foci differing from the nuclear bodies containing the 90 kDa protein. In electron microscopy analysis, the intranuclear foci containing the 80 kDa MFP1 appeared as small loose structures at the periphery of condensed chromatin patches. This protein was also located in the nucleolus. It was abundant in meristematic cells, but its level fell when proliferation stopped. This different expression and distribution, and its preferential location at the boundaries between heterochromatin and euchromatin, suggest that the novel 80 kDa protein might be associat...Continue Reading

References

Aug 22, 2003·Nucleic Acids Research·Sun Yong JeongIris Meier
May 4, 2004·Experimental Cell Research·Luis A ParadaTom Misteli
Dec 18, 2004·Plant Molecular Biology·Satoru FujimotoKiichi Fukui
Jan 19, 2005·Cell Cycle·Peter Shaw, John Doonan
Nov 18, 2005·Journal of Experimental Botany·Rafael SamaniegoSusana Moreno Díaz de la Espina
Dec 7, 2005·Planta·Rafael SamaniegoSusana Moreno Díaz de la Espina
May 12, 2006·Current Opinion in Cell Biology·Thomas CremerStanislav Fakan
Dec 25, 2007·Cell Biology International·José R CruzSusana Moreno Díaz de la Espina

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Citations

Mar 17, 2009·Trends in Plant Science·Kirsten Krause, Karin Krupinska
Mar 19, 2016·Biochimica Et Biophysica Acta·Joanna MelonekKarin Krupinska
Dec 2, 2010·Biology of the Cell·Clara Pérez-Munive, Susana Moreno Díaz de la Espina
Jun 23, 2009·Psychotherapy Research : Journal of the Society for Psychotherapy Research·Howard SteeleAnne Murphy
May 18, 2018·Attachment & Human Development·Christin KöberTilmann Habermas
Oct 12, 2010·Attachment & Human Development·Amy L Busch, Alicia F Lieberman
Dec 24, 2011·Attachment & Human Development·Jude CassidyJason D Jones
Oct 31, 2012·Attachment & Human Development·Sonia GojmanGerardo Hernández
Oct 31, 2012·Attachment & Human Development·Madelon M E RiemSerge A R B Rombouts

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