PMID: 9185706Jun 11, 1997Paper

Characterization of 10 new monoclonal antibodies against prostate-specific antigen by analysis of affinity, specificity and function in sandwich assays

International Journal of Cancer. Journal International Du Cancer
E CoreyR L Vessella

Abstract

While prostate-specific antigen (PSA) is already an invaluable marker for prostate cancer, there is continuing demand for new anti-PSA antibodies with specific characteristics, e.g., high sensitivity and specificity and equimolar binding to free PSA (f-PSA) and the PSA-alpha-1-antichymotrypsin complex (PSA-ACT), as well as the ability to distinguish between these 2 immunoreactive forms of PSA. We have therefore generated and characterized 10 anti-PSA monoclonal antibodies (MAbs). Apparent dissociation constants (Kd) of MAbs were determined by direct ELISA yielding Kd-0.2-164.0 nM. Western blots suggested that 3 of the MAbs (60-1A2, 60-8A2 and 17-1A2) bind to linear epitopes. Sandwich assays identified 5 major antigenic regions as binding targets of the MAbs. Three combinations of MAbs recognize f-PSA and PSA-ACT in equimolar fashion with high sensitivity. Two of the MAb combinations are specific for f-PSA. Physical analysis of the new antibodies has allowed us to assign the MAbs to binding classes (based on their sandwiching capabilities) and to determine accurate apparent dissociation constants.

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Citations

Jul 27, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·B G BlijenbergF H Schröder
Oct 6, 1998·Critical Reviews in Clinical Laboratory Sciences·H G RittenhouseA W Partin
Aug 4, 1998·International Journal of Cancer. Journal International Du Cancer·E Corey, M J Corey
Apr 25, 2000·The Journal of Biological Chemistry·M J CoreyR L Vessella
Sep 1, 2015·Chemical Science·Alexander Stephenson-BrownPaula M Mendes
May 9, 2002·International Journal of Pharmaceutics·Priyabrata SarkarIbtisam E Tothill
Jan 19, 2000·Clinical Biochemistry·B G BlijenbergF H Schröder

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