PMID: 3755179Jan 1, 1986Paper

Characterization of 1,25-dihydroxyvitamin D3-dependent calcium uptake in isolated chick duodenal cells

The Journal of Membrane Biology
C T LiangB Sacktor

Abstract

The in vivo and in vitro effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on calcium uptake by isolated chick duodenal cells were studied. In vivo, 1,25-(OH)2D3 given orally to vitamin D-deficient chicks increased the initial rate of calcium uptake by cells prepared 1 hr after administration of the hormone. The rate was stimulated approximately 100%, 17 to 24 hr after repletion. In vitro, pre-incubation of 1,25-(OH)2D3 with cells from D-deficient chicks increased the cellular rate of calcium uptake in a concentration-dependent relationship. Enhancement was found with 10(-15) M, was maximal at 10(-13) M, and was diminished at higher (10(-11) M) concentrations. Stimulation was observed after a pre-incubation period as brief as 1 hr. The potency order for vitamin D3 analogs was 1,25-(OH)2D3 = 1-(OH)D3 greater than 25-(OH)D3 greater than 1,24,25-(OH)3D3 greater than 24,25-(OH)2D3 greater than D3. The maximal enhancement in calcium uptake induced by the analogs was the same, only the concentration at which the cell responded was different. The effectiveness of 1,25-(OH)2D3 was five orders of magnitude greater than D3. Kinetically, 1,25-(OH)2D3 increased the Vmax of calcium uptake; the affinity for calcium (Km = 0.54 mM) was unchan...Continue Reading

References

Jun 1, 1975·Archives of Biochemistry and Biophysics·S Moriuchi, H F Deluca
Sep 1, 1983·Journal of Cellular Physiology·F BronnerJ H Lipton
May 1, 1982·The American Journal of Physiology·C T LiangB Sacktor
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·O FontaineH Rasmussen
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C T LiangB Sacktor
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R H WassermanC S Fullmer
Feb 1, 1959·The American Journal of Physiology·D SCHACHTER, S M ROSEN

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Citations

Feb 1, 1989·Pflügers Archiv : European journal of physiology·P A LucasT Drüeke
Jun 24, 1987·Biochimica Et Biophysica Acta·C H van Os
Nov 13, 1991·Biochimica Et Biophysica Acta·P Proulx
Apr 23, 1990·Biochimica Et Biophysica Acta·S TakamotoC T Liang
Dec 29, 2015·Archives of Biochemistry and Biophysics·Valeria A RodríguezNori G Tolosa de Talamoni
Apr 1, 1993·Bone and Mineral·B H ArjmandiD N Kalu
Aug 7, 1987·Biochimica Et Biophysica Acta·T OhyashikiT Mohri
Nov 6, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Viviana A CentenoNori G Tolosa de Talamoni
Feb 13, 2010·The Journal of Nutritional Biochemistry·Adriana PérezNori G Tolosa de Talamoni
May 9, 2002·Biochimica Et Biophysica Acta·Vivek Kumar, Rajendra Prasad
Feb 13, 2007·Biochimica Et Biophysica Acta·Kaler PawanPrasad Rajendra

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