Characterization of 14-3-3 isoforms expressed in the Echinococcus granulosus pathogenic larval stage

Journal of Proteome Research
Aline TeichmannHenrique B Ferreira

Abstract

The 14-3-3 protein family of eukaryotic regulators was studied in Echinococcus granulosus, the causative agent of cystic hydatid disease. These proteins mediate important cellular processes in eukaryotes and are expected to play important roles in parasite biology. Six isoforms of E. granulosus 14-3-3 genes and proteins (Eg14-3-3.1-6) were analyzed, and their phylogenetic relationships were established with bona fide 14-3-3 orthologous proteins from eukaryotic species. Eg14-3-3 isoforms with previous evidence of expression (Eg14-3-3.1-4) in E. granulosus pathogenic larval stage (metacestode) were cloned, and recombinant proteins were used for functional studies. These protein isoforms were detected in different components of E. granulosus metacestode, including interface components with the host. The roles that are played by Eg14-3-3 proteins in parasite biology were inferred from the repertoires of interacting proteins with each isoform, as assessed by gel overlay, cross-linking, and affinity chromatography assays. A total of 95 Eg14-3-3 protein ligands were identified by mass spectrometry. Eg14-3-3 isoforms have shared partners (44 proteins), indicating some overlapping functions; however, they also bind exclusive partners (5...Continue Reading

References

Jul 17, 1998·Current Opinion in Structural Biology·C B Burge, S Karlin
Apr 26, 2000·Genome Research·A A Salamov, V V Solovyev
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·S P GygiR Aebersold
Sep 30, 2000·Parasite Immunology·M Siles-LucasM Breijo
Feb 28, 2003·The EMBO Journal·Martin WürteleClaudia Oecking
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
May 27, 2004·The Journal of Biological Chemistry·Sarah E M MeekHelen Piwnica-Worms
Jun 28, 2005·Nucleic Acids Research·John Besemer, Mark Borodovsky
Aug 11, 2005·Science's STKE : Signal Transduction Knowledge Environment·Dave Bridges, Greg B G Moorhead
Feb 14, 2006·Proteomics·Ross D Alexander, Peter C Morris
Jun 23, 2006·Current Pharmaceutical Biotechnology·E KjarlandR Kleppe
Oct 13, 2006·Journal of Cellular Biochemistry·R ParedesN Galanti
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiaowen YangJonathan M Elkins
Nov 17, 2006·Molecular and Cellular Biochemistry·Ivan S ChernikNikolai B Gusev
Oct 30, 2007·Protein Science : a Publication of the Protein Society·Fred P DavisAndrej Sali
Mar 5, 2008·Experimental Parasitology·Mar Siles-LucasBruno Gottstein
Mar 11, 2008·Experimental Parasitology·Alessandra SiracusanoAntonella Teggi
Oct 19, 2010·Current Biology : CB·Qiongqiong ZhouDouglas N Robinson
Jan 25, 2011·Trends in Parasitology·Alvaro DíazFernando Ferreira
Jul 20, 2011·Journal of Proteome Research·Kirby N SwatekJay J Thelen
Aug 20, 2011·PloS One·Stephen J BrokxRaymond Hui
Aug 27, 2011·Journal of Molecular Modeling·Marbella Maria da FonsêcaAna Tereza Ribeiro Vasconcelos
Sep 6, 2011·Seminars in Cell & Developmental Biology·Rune KleppeJan Haavik
Sep 17, 2011·Seminars in Cell & Developmental Biology·Tomas Obsil, Veronika Obsilova
Sep 29, 2011·Seminars in Cell & Developmental Biology·Alexandra K Gardino, Michael B Yaffe
Dec 14, 2011·The FEBS Journal·Manuela MolzanChristian Ottmann
Jan 21, 2012·Molecular and Biochemical Parasitology·Veridiana G VirginioHenrique B Ferreira
Mar 29, 2012·Journal of Proteome Research·Marco LalleEdoardo Pozio

❮ Previous
Next ❯

Citations

Feb 14, 2016·Veterinary Parasitology·Christian HidalgoRodolfo Paredes
Apr 27, 2017·Journal of Proteomics·Karina M MonteiroHenrique B Ferreira
Jan 8, 2019·PLoS Neglected Tropical Diseases·María Celeste NicolaoAndrea C Cumino
Nov 6, 2015·BMC Microbiology·Patricia Akemi AssatoAna Marisa Fusco-Almeida

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.