Characterization of 17beta-hydroxysteroid dehydrogenase isoenzyme expression in benign and malignant human prostate

International Journal of Cancer. Journal International Du Cancer
J P EloR K Vihko

Abstract

In the present study, expressions of 17beta-hydroxysteroid dehydrogenase (17HSD) types 1, 2, and 3, 5alpha-reductase type 2 and human androgen receptor mRNAs were determined in 12 benign prostatic hyperplasia and 17 prostatic carcinoma specimens. 17HSD type 2 was found to be the principle isoenzyme expressed in the prostate. Significantly higher expressions of 17HSD type 2 and 5alpha-reductase type 2 were detected in benign prostatic hyperplasia compared with the carcinoma specimens. Expression of the androgen receptor in the 2 groups was not significantly different. 17HSD type 3 mRNA was not detected in any of the specimens investigated. Only low constructive expression of the 2.3 kb mRNA of 17HSD type 1 was seen. Immunohistochemical analysis indicated that this did not lead to significant enzyme expression, only faint staining for the enzyme protein being detected, mainly in uroepithelial cells. No significant correlation was found between any of the mRNAs analysed, but the data on 5alpha-reductase type 2 mRNA support the presence of an increased proportion of 5alpha-dihydrotesterone in the hyperplastic prostate. In cultured PC-3 prostatic cancer cells and in the transiently transfected human embryonic kidney 293 cells, 17HSD...Continue Reading

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Citations

Nov 29, 2005·PLoS Genetics·Peter KraftSholom Wacholder
Nov 7, 2006·The Journal of Steroid Biochemistry and Molecular Biology·R MindnichJ Adamski
Oct 24, 2013·The Prostate·Mark A TitusJames L Mohler
Jul 12, 2002·The Prostate·Ann W HsingFrank Z Stanczyk
Jan 25, 2005·The Journal of Steroid Biochemistry and Molecular Biology·P SoronenP Vihko
Mar 28, 2007·Prostate Cancer and Prostatic Diseases·K SuzukiF Labrie
Mar 20, 1998·International Journal of Cancer. Journal International Du Cancer·S DélosP M Martin
Dec 22, 1999·Breast Cancer Research and Treatment·M MiettinenP Vihko
May 17, 2017·Bioorganic & Medicinal Chemistry Letters·Anna VuorinenBarbara Matuszczak
Feb 13, 2001·Molecular and Cellular Endocrinology·P VihkoD Ghosh
Feb 13, 2001·Molecular and Cellular Endocrinology·D PoirierV Luu-The
Feb 13, 2001·Molecular and Cellular Endocrinology·B HusenA Einspanier
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·N Moghrabi, S Andersson
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·P VihkoV Isomaa
Mar 18, 2004·Molecular and Cellular Endocrinology·P VihkoV Isomaa
May 8, 2004·Molecular and Cellular Endocrinology·R MindnichJ Adamski

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