PMID: 707382Oct 1, 1978

Characterization of 7-alpha-dehydroxylase in Clostridium leptum

The American Journal of Clinical Nutrition
E J Stellwag, Phillip B Hylemon


7-alpha-Dehydroxylation of primary bile acids was demonstrated radiochromatographically in whole cells of Clostridium leptum but was not observed in intestinal Bacteroides species. Activity of 7-alpha-Dehydroxylase was detected within a pH range of 5 to 9 and was 8-fold higher in specific activity in cell cultures in the presence of 0.1 mM sodium cholate than in its absence. 7-alpha-Dehydroxylase activity in whole cells was markedly inhibitied by 2,4-dinitrophenol, carbonyl-cyanide-m-chlorophenylhydrazine, and dicyclohexylcarbodiimide. A hypothesis concerning the dietary regulation of 7-alpha-dehydroxylating intestinal anaerobic bacteria is presented.


Jan 1, 1995·Critical Reviews in Microbiology·B K Mital, S K Garg
Mar 2, 2016·Therapeutic Advances in Gastroenterology·Shaan GuptaElaine O Petrof
Jan 1, 1987·Nutrition and Cancer·J Roberts-AndersenR B Wilson
Jan 1, 1991·Die Nahrung·D W Bleyl
Nov 29, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alexa R WeingardenAlexander Khoruts
May 21, 2016·PloS One·Marialena MouzakiJohane P Allard
Nov 22, 2005·Journal of Lipid Research·Jason M RidlonPhillip B Hylemon

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Bile Salts
Carbonyl Cyanide m-Chlorophenyl Hydrazone
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