PMID: 9431996Feb 7, 1998Paper

Characterization of a 200-kDa diatom protein that is specifically associated with a silica-based substructure of the cell wall

European Journal of Biochemistry
Nils KrögerManfred Sumper

Abstract

The cell wall of a diatom is made up of a silica-based scaffold and organic macromolecules. Proteins located in the cell wall are believed to control morphogenesis of the species-specific silica structures of the scaffold. However, data that correlate distinct silica elements and specific proteins within the diatom cell wall have not been reported. Here, the cell wall protein HEP200 (200-kDa HF-extractable protein) from the diatom Cylinidrotheca fusiformis is identified and characterized. HEP200 is tightly associated with a substructure of the silica scaffold. It is a member of a new protein family, of which two more members are identified. Each member displays the same bipartite structure. The N-terminal part consists of a variable number of a repeated sequence motif (PSCD domain), whereas the C-terminal part is unique. Immunolocalization experiments revealed the arrangement of different proteins within the cell wall. Frustulins, a previously described group of glycoproteins, constitute the outer coat of the cell wall and exhibit a ubiquitous distribution. In contrast, HEP200 is specifically located at a subset of about six silica strips in intact cell walls, shielded by frustulins. This study therefore identifies a diatom cel...Continue Reading

References

Oct 1, 1977·Analytical Biochemistry·A J Mort, D T Lamport
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jul 15, 1996·European Journal of Biochemistry·N KrögerM Sumper
May 1, 1964·Experimental Cell Research·B E REIMANN

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May 24, 2008·Natural Product Reports·Heinz C SchröderWerner E G Müller
Apr 12, 2011·Chemical Communications : Chem Comm·Siddharth V Patwardhan
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·David KisailusDaniel E Morse
Sep 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nicole PoulsenNils Kröger
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·K ShimizuD E Morse
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Emanuel Epstein
Sep 12, 2002·Annual Review of Plant Biology·Angela Falciatore, Chris Bowler
Nov 6, 2008·Annual Review of Genetics·Nils Kröger, Nicole Poulsen
Jan 1, 2012·Scientifica·Daniel Otzen
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Jan 16, 2016·Biotechnology for Biofuels·Yoshiaki MaedaTsuyoshi Tanaka
Nov 10, 2007·Phytochemistry·Tomás Rezanka, Karel Sigler
Oct 20, 2004·Trends in Biotechnology·Cheryl Wong Po FooDavid L Kaplan
Jun 17, 2016·Nature Communications·Michael J KnightPaul Curnow
Jul 29, 2015·Biomaterials Science·Carolin C Lechner, Christian F W Becker
May 28, 2014·Chemistry : a European Journal·Ren-Hua JinRumi Tamoto Levi
Oct 14, 2006·Macromolecular Bioscience·Scott A MillerDavid Wright
Jan 3, 2019·Chemistry : a European Journal·Ren-Hua Jin
Sep 29, 2005·Physical Chemistry Chemical Physics : PCCP·Katharina LutzEike Brunner
Jan 8, 2020·Organic & Biomolecular Chemistry·Fabian DausArmin Geyer
Jan 31, 2003·The Journal of Biological Chemistry·Alexander HeissWilli Jahnen-Dechent
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Feb 14, 2007·Macromolecular Bioscience·Alexandre Mantion, Andreas Taubert
Feb 1, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Carolin C Lechner, Christian F W Becker
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