PMID: 12770184May 29, 2003Paper

Characterization of a 212kD protein, released into the host by the larva of the endoparasitoid Chelonus inanitus (Hymenoptera, Braconidae)

Journal of Insect Physiology
A Hochuli, Beatrice Lanzrein

Abstract

Previous studies have shown that the larva of Chelonus inanitus plays an essential role in inducing the precocious onset of metamorphosis in polydnavirus/venom-containing Spodoptera littoralis, and that this effect might be due to proteins released by the parasitoid larva into the host. Here we show that a 212kD protein is the predominant protein released in vitro by late first instar parasitoids. This protein does not accumulate anywhere in the parasitoid body. Polyclonal antibodies specific for the 212kD protein were produced and immunoblot analyses revealed that this protein begins to be released by parasitoid larvae when the precocious onset of metamorphosis is induced in the host. Amino acid sequences of the N-terminus and of internal fragments of the 212kD protein were determined and glycoprotein detection showed that this protein is glycosylated. The availability of specific antibodies and the amino acid sequence information should now allow us to clone and express the 212kD protein and to directly study its functional significance.

References

Jan 1, 1994·Insect Biochemistry and Molecular Biology·A I Soldevila, D Jones
Jan 1, 1993·Archives of Insect Biochemistry and Physiology·A I Soldevila, D Jones
Mar 29, 2001·Archives of Insect Biochemistry and Physiology·E H Richards, J P Edwards
May 29, 2003·Journal of Insect Physiology·J J. Brown, D A. Reed

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Citations

Jan 31, 2006·Bulletin of Entomological Research·M H Greenstone
Dec 4, 2003·Annual Review of Entomology·Jeremy A Kroemer, Bruce A Webb
May 19, 2009·Journal of Insect Physiology·Rita Pfister-Wilhelm, Beatrice Lanzrein
Jul 9, 2005·Journal of Insect Physiology·E M HegaziL M Z Abo Abd-Allah
Aug 2, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·F L CônsoliS B Vinson
Sep 26, 2006·Advances in Virus Research·Torrence A GillBruce A Webb

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