PMID: 8944761Nov 1, 1996Paper

Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas testosteroni

European Journal of Biochemistry
U C Oppermann, E Maser

Abstract

A new form of the NAD(P)-dependent 3 alpha-hydroxysteroid dehydrogenases (3 alpha-HSDs), present in the gram-negative bacterium Comamonas testosteroni ATCC 11996, was isolated from a testosterone-induced bacterial extract and characterized. The enzyme (HSD 28) has a monomeric molecular mass of 28 kDa. It belongs to the protein superfamily of short-chain dehydrogenases/reductases (SDR) as established by N-terminal sequence analysis. Along with the 3 alpha-hydroxysteroid dehydrogenase and 3-oxo-reductase activities towards a variety of cis or trans fused A/B ring steroids, it also reduces several xenobiotic carbonyl compounds, including a metyrapone-based class of insecticides, to the respective alcohol metabolites. No dihydrodiol dehydrogenase activity towards trans- or cis-benzene-dihydrodiols could be detected, thus distinguishing it from the indomethacine-sensitive, mammalian liver type 3 alpha-HSDs. Subcellular fractionation revealed that the enzyme is localized in the cytoplasm of the bacterial cell. Proteins similar to the 3 alpha-HSD were detected and characterized from Comamonas testosteroni strain ATCC 17454 and from a commercially available steroid-induced extract of a patent Pseudomonas strain. The N-terminal amino ac...Continue Reading

References

Oct 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·J W Funder
Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·U C OppermannK J Netter
Jul 1, 1989·Endocrinology·D M PhillipsC Monder
Oct 1, 1988·Applied and Environmental Microbiology·E García-ValdésJ Ursing
Jan 1, 1986·Biochemical Pharmacology·I R Rowland
Jan 1, 1987·Reviews of Infectious Diseases·D J BarbaroP M Southern
Aug 1, 1974·Journal of Steroid Biochemistry·M Watanabe, H Watanabe
Oct 1, 1982·Analytical Biochemistry·G L GertonJ L Hedrick
Nov 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·J H AbalainH H Floch
May 9, 1995·Biochemistry·H JörnvallD Ghosh
Nov 1, 1994·Molecular and Cellular Endocrinology·A L AlbistonZ S Krozowski
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·S S HoogM Lewis

❮ Previous
Next ❯

Citations

Nov 23, 2010·Applied Microbiology and Biotechnology·Bodo Philipp
Jun 12, 2002·Biochemical and Biophysical Research Communications·Dirk SkowaschEdmund Maser
Jan 1, 1997·Brain Research Bulletin·F CelottiA Poletti
Nov 28, 2007·Journal of Bacteriology·Yin-Ru ChiangGeorg Fuchs
Aug 19, 2014·Journal of Biotechnology·Thorsten EggertWerner Hummel
Jun 12, 2012·The Journal of Steroid Biochemistry and Molecular Biology·Wenjie GongEdmund Maser
Nov 30, 2004·Biochemical and Biophysical Research Communications·Guo-Hua ZhangTie-An Xia
Jan 18, 2015·Chemico-biological Interactions·Yuanhua YuHao Zhang
Mar 10, 2017·Chemico-biological Interactions·Ye JiYuanhua Yu
Aug 9, 2003·The Journal of Pharmacy and Pharmacology·Barbora SzotákováVladimír Wsól
Aug 4, 2019·Applied and Environmental Microbiology·Masae HorinouchiToshiaki Hayashi
Apr 22, 2008·The Journal of Biological Chemistry·André GöhlerEdmund Maser
Jan 13, 2001·Chemico-biological Interactions·G L Forrest, B Gonzalez
Aug 28, 2021·Microorganisms·Franziska Maria FellerBodo Philipp
Jun 19, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Edmund Maser, Guangming Xiong
Nov 9, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Masae HorinouchiToshiaki Kudo
Sep 3, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Michael KisielaEdmund Maser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.