PMID: 9556407Oct 1, 1996Paper

Characterization of a 4.2-kb plasmid isolated from periodontopathic spirochetes

Oral Microbiology and Immunology
E C ChanR Siboo

Abstract

Oral anaerobic treponemes are assoicated with active periodontal disease and may comprise up to 57% of the microbiota in periodontal pockets. Four treponeme strains (designated U2a, U2b, U9b, and U9c) isolated from clincial cases were found to harbor a new 4.2-kb plasmid when plasmid DNA was extracted and purified employing the Qiagen Plasmid Kit. This plasmid differs from the smaller plasmids (2.0-, 2.6-, and 2.7-Kb) reported previously by others in Treponema denticola. The newly discovered 4.2-kb plasmid was found to be the same in all four treponeme strains by restriction endonuclease analysis. It is a circular plasmid since restriction with PstI, Pvu II, Sma I, Xma I, Ava 1 or Bam HI produced a single band of the same size. Bacterial strain U2b was shown to be Treponema socranskii and U9c to be T. denticola. The plasmid is designated "pTS1". The presence of the same plasmid in different species of the treponemes isolated from the same patient suggests the possibility of a naturally occurring genetic transfer system within the oral spirochetes or their ability to take up and maintain mobilizable plasmids.

References

Mar 1, 1991·FEMS Microbiology Letters·A IvicC W Penn
Nov 1, 1988·Advances in Dental Research·W J Loesche
Apr 1, 1988·Infection and Immunity·L G SimonsonH E Morton
Dec 1, 1980·Journal of Clinical Microbiology·S B Leschine, E Canale-Parola
Feb 1, 1995·Oral Microbiology and Immunology·S CaudryE C Chan
Oct 1, 1994·Oral Microbiology and Immunology·E ReedyL Zablen
Apr 1, 1993·Oral Microbiology and Immunology·E C ChanA Klitorinos

❮ Previous
Next ❯

Citations

Jan 9, 2004·Plasmid·Sarita Chauhan, Howard K Kuramitsu
Sep 22, 2011·Expert Reviews in Molecular Medicine·Judith Miklossy
Mar 31, 2011·Journal of Dental Research·J R FrederickR T Marconi
Aug 5, 2006·Anaerobe·J C Fenno, B C McBride
Aug 9, 2005·FEMS Microbiology Letters·Mitsunori YamadaHoward K Kuramitsu
Mar 19, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M B Visser, R P Ellen
Oct 6, 2000·Annual Review of Microbiology·P E Kolenbrander
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Howard K Kuramitsu
Jul 15, 2015·Applied and Environmental Microbiology·Valentina GodovikovaJ Christopher Fenno
Apr 4, 2002·Oral Microbiology and Immunology·B Y WangH K Kuramitsu
Jun 7, 2013·Journal of Oral Microbiology·Ingar OlsenBing-Yan Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.