PMID: 7547685Apr 1, 1995Paper

Characterization of a 50 kDa surface membrane protein on thymic stromal cells as an important factor for early T cell development

International Immunology
T TakeuchiHiroshi Yamamoto

Abstract

We previously reported that the nude mouse-derived splenic T cell clone, N-9F, exhibits a proliferative response to the SL10.3 thymic epithelial cell clone. In the present study we generated an Armenian hamster mAb, HS9, specific for SL10.3, which inhibited the N-9F's proliferative response to SL10.3. We performed thymocyte repopulation experiments using fetal liver cells and 2'-deoxyguanosine-treated thymic rudiments. After 14 days of culture, donor fetal liver cells proliferated and differentiated to CD4+CD8+ and CD4-CD8+ with some CD4+CD8- cells in the host thymic rudiments. However, most of the thymocytes remained at a CD4-CD8- immature stage in the presence of HS9 and the cell recovery was reduced to 30% of the control. Immunohistostaining and flow cytometry studies revealed that HS9 reacted with stromal cells of fetal thymus at the earliest from day 14 gestation. Neither thymocytes nor lymph node T cells were stained with HS9. HS9 antigen was distributed not only on thymic subcapsular and cortical stromal cells, but also on peripheral B cells in adult mice. The antigen that HS9 detected was found to be a 50 kDa surface membrane protein on thymic stromal cells. On the other hand, the 50 kDa molecule is associated with two ...Continue Reading

Citations

Jan 1, 1996·Annual Review of Immunology·G AndersonE J Jenkinson
Mar 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasuhiro Kohama
Aug 1, 1997·The Tohoku Journal of Experimental Medicine·M SaitoM Matsuyama

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