Characterization of a bacterial beta-1,3-galactosyltransferase with application in the synthesis of tumor-associated T-antigen mimics

Biochemistry
Wen YiPeng George Wang

Abstract

T-Antigen (Gal-beta1,3-GalNAc-alpha-O-Ser/Thr) is an important precursor of mucin-type O-glycans. T-Antigen is found to be closely associated with cancer progression and metastasis and has been used to develop carbohydrate-based anticancer vaccines. Enzymatic synthesis of T-antigen disaccharides have relied on the use of beta-1,3-galactosyltransferases recently cloned and characterized from several eukaryotic organisms. However, its application is limited by the difficulty of obtaining homogeneous enzymes and the strict substrate specificity of enzymes. Recently, a number of bacteria have been found to express carbohydrate structures that mimic host glycans. The corresponding glycosyltransferases have been exploited in the facile synthesis of a number of clinically important glycoconjugate mimics. In this study, we biochemically characterized a bacterial beta-1,3-galactosyltransferase (WbiP) from Escherichia coli O127, which expresses a T-antigen mimic in the lipopolysaccharide (LPS) structure. Substrate study showed that WbiP could readily glycosylate a series of N-acetylgalactosamine (GalNAc) analogues with alpha-substitutions at the reducing end, including glycosylated Ser and Thr (GalNAc-alpha-O-Ser/Thr), which illustrates ...Continue Reading

References

Aug 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·G F Springer
Jun 27, 2002·Biochemical and Biophysical Research Communications·Jun ShaoPeng George Wang
Jun 29, 2004·International Journal of Cancer. Journal International Du Cancer·John SotiriadisYoon Berm Kim

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Citations

Aug 31, 2010·Biochemical and Biophysical Research Communications·Nicholas PettitPeng George Wang
Nov 5, 2014·Frontiers in Immunology·Inka Brockhausen
Feb 25, 2018·Applied Microbiology and Biotechnology·Rachel Chen
Oct 10, 2008·Journal of the American Chemical Society·Wen YiPeng George Wang

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