Characterization of a bacterial gene encoding an autophosphorylating protein tyrosine kinase

Gene
C GrangeasseA J Cozzone

Abstract

Acinetobacter johnsonii harbors a protein tyrosine kinase activity that is able to catalyze autophosphorylation, like a number of eukaryotic tyrosine kinases. A biochemical and genetic analysis of this enzyme was performed. Maximum phosphorylation in vitro was obtained by incubating the kinase for 2 min at pH 7.0 in the presence of 5 mM magnesium chloride. In contrast to eukaryotic enzymes, no inhibitory effect of genistein and no phosphorylation of synthetic substrates such as poly (Glu80 Tyr20) or angiotensin II were observed. The analysis of the bacterial kinase by two-dimensional gel electrophoresis revealed the presence of at least five isoforms, all phosphorylated exclusively at tyrosine, which supports the concept that autophosphorylation occurs at multiple sites within the protein. The cloning and nucleotide sequencing of the gene encoding this kinase were achieved, which represents the first molecular characterization of a gene of this type in bacteria. An open reading frame of 2199 nucleotides encoding a protein of 82,373 Da was detected. The analysis of the deduced amino acid sequence suggested a possible involvement of the enzyme in cell recognition and bacterial pathogenicity. In addition, the cloning and sequencin...Continue Reading

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Citations

Aug 21, 2003·Biochemical and Biophysical Research Communications·Virginie MolleJean-François Prost
Jul 31, 1999·FEMS Microbiology Letters·C GrangeasseE Godfroid
Dec 22, 1999·Trends in Cell Biology·C J Bakal, J E Davies
Dec 18, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R PrenetaA J Cozzone
Aug 15, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christophe GrangeasseIvan Mijakovic
Jan 18, 2006·Journal of Molecular Microbiology and Biotechnology·Alain J Cozzone
Dec 4, 2013·Antioxidants & Redox Signaling·Alistair J Standish, Renato Morona
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J WuA Newton
Apr 15, 2014·Genome Biology and Evolution·Lei ShiIvan Mijakovic
Jan 13, 1999·Molecular Plant-microbe Interactions : MPMI·G CondemineC Enard
Nov 26, 2003·Biochemical and Biophysical Research Communications·Leonilde M MoreiraIsabel Sá-Correia
Sep 1, 2009·Biochimica Et Biophysica Acta·Christophe GrangeasseSylvie Nessler
Jul 14, 2007·FEMS Microbiology Letters·Didier SoulatChristophe Grangeasse
May 1, 2016·Trends in Microbiology·Christophe Grangeasse
Jan 9, 2007·Trends in Biochemical Sciences·Christophe GrangeasseIvan Mijakovic
Jul 26, 2002·The Journal of Biological Chemistry·Patricia DoubletAlain J Cozzone
Dec 26, 2001·The Journal of Biological Chemistry·Christophe GrangeasseAlain J Cozzone
Apr 2, 2013·The Journal of Biological Chemistry·Deniz B TemelRanajeet Ghose
Feb 21, 2014·The Journal of Biological Chemistry·Joseph D ChaoYossef Av-Gay
Oct 19, 2001·The Journal of Biological Chemistry·M H Bender, J Yother

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