Characterization of a bi-directional promoter for divergent transcription of a PHD-zinc finger protein gene and a ran gene in the protozoan pathogen Giardia lamblia

Molecular Microbiology
Shiou-Jeng OngJung-Hsiang Tai

Abstract

We showed previously that transcription of the ran gene in Giardia lamblia is regulated by an AT-rich initiator. In the present study, the ran initiator was found to regulate transcription of a neighbouring PHD zinc-finger protein gene. Deletion and scanning mutagenesis of the phd promoter in a firefly luciferase reporter system showed that the promoter activity is determined by multiple single-stranded T-tract DNA elements distributed into a distal domain spanning the ran initiator (-134/-103) and a proximal domain (-88/-48) spanning phd messenger RNA (mRNA) start sites (-74, -55 and -53 relative to the first ATG). The promoter activity is repressed by the single T-tract element on a non-template strand of the ran initiator, and is activated by closely spaced T-tract elements on the opposite strand. The T-tract elements in the phd and ran initiators compete for similar ssDNA binding proteins. Mutation of -47/-42 resulted in dramatic reduction of luciferase activity without changing luciferase mRNA levels, indicating the potential involvement of a regulatory mechanism in PHD protein translation. These findings suggest that G. lamblia uses multiple copies of a T-tract element as both core and distal elements in regulating transc...Continue Reading

References

Dec 1, 1991·Microbiological Reviews·R D Adam
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·D B Keister
Mar 1, 1995·Molecular Microbiology·M R MowattH H Stibbs
Feb 1, 1995·Trends in Biochemical Sciences·R AaslandA F Stewart
May 1, 1994·Journal of Cellular Biochemistry·T Boulikas
Apr 1, 1993·Molecular and Biochemical Parasitology·P L EyG Mayrhofer
Jun 1, 1996·Molecular and Biochemical Parasitology·J E PurdyW A Petri
Jan 1, 1996·Annual Review of Microbiology·F D GillinJ M McCaffery
Jun 1, 1997·Trends in Biochemical Sciences·A B Sachs, S Buratowski
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·U SinghW A Petri
May 9, 1998·Molecular and Biochemical Parasitology·C H SunJ H Tai
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·M Hampsey
Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Upcroft, P Upcroft
Mar 25, 2000·International Journal for Parasitology·R D Adam
Oct 29, 2000·Cell·A J Berk
Mar 20, 2001·Molecular and Biochemical Parasitology·H G ElmendorfT E Nash

❮ Previous
Next ❯

Citations

Apr 3, 2003·International Journal for Parasitology·Stepanka VanacovaPatricia J Johnson
Apr 27, 2005·Molecular and Biochemical Parasitology·Hannah E J Polson, Michael J Blackman
Oct 27, 2011·PLoS Neglected Tropical Diseases·Wei LiChing C Wang
Dec 5, 2020·International Journal for Parasitology·Samantha J Emery-CorbinAaron R Jex

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.