Characterization of a C alpha gene of swine

Molecular Immunology
W R Brown, J E Butler

Abstract

The cDNA sequence encoding the constant region of the porcine IgA heavy chain as well as the exon-intron structure of the germline gene, have been determined. A cDNA clone (1A1) spanning the CH3 domain and part of the CH2 domain was isolated from a porcine mesenteric lymph node cDNA library. Clone 1A1 was aligned with a PCR-generated DNA fragment encompassing the CH1 domain through the 5' end of the CH3 domain to derive the complete cDNA sequence. Comparison with other mammalian C alpha heavy chains (hinge regions excluded) indicated that the deduced amino acid sequence of porcine C alpha is most homologous with the human C alpha subclasses (> 70%), followed by mouse C alpha (61%) and a consensus sequence of the 13 rabbit C alpha heavy chains (59%). The greatest sequence homology was found among the CH3 domains in all species. A striking feature of porcine C alpha is its short six amino acid hinge which like other mammalian IgAs, is encoded with the CH2 domain. Sequence analysis of germline C alpha, generated by PCR from liver or sperm DNA, revealed an exon-intron organization similar to other mammalian C alpha genes. Genomic Southern blot data are consistent with the presence of a single C alpha gene within the porcine genome....Continue Reading

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Citations

Nov 1, 1996·Veterinary Immunology and Immunopathology·J E ButlerP Navarro
Apr 9, 1998·Veterinary Immunology and Immunopathology·B WagnerA Radbruch
May 1, 1997·Immunology·W R BrownL Hammarström
Jun 26, 2002·Molecular Biology and Evolution·Kenta SumiyamaShintaroh Ueda
Sep 24, 2004·Immunology·Jenny M Woof, Michael A Kerr
Dec 17, 2014·Retrovirology·Mingkui Zhou, Ruth M Ruprecht
Sep 20, 2005·Developmental and Comparative Immunology·John E ButlerMarek Sinkora
Sep 23, 2008·Developmental and Comparative Immunology·J E ButlerI Kacskovics
Apr 14, 2006·Veterinary Research·Veerle SnoeckEric Cox
Jan 29, 2010·Clinical and Vaccine Immunology : CVI·Juan M PachecoWilliam T Golde
Nov 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·John E ButlerKevin Wells
Apr 29, 1998·Journal of Virology·I SolaL Enjuanes

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