Characterization of a c-type heme-containing PAS sensor domain from Geobacter sulfurreducens representing a novel family of periplasmic sensors in Geobacteraceae and other bacteria

FEMS Microbiology Letters
Yuri Y LonderMarianne Schiffer

Abstract

Geobacter sulfurreducens encodes one of the largest numbers of proteins annotated as parts of the two-component signal transduction and/or chemotaxis pathways. Ten of these signal transducers have homologous periplasmic sensor domains that contain the sequence signature for c-type hemes. One such sensor domain encoded by gene GSU0303 was isolated and characterized. The protein was expressed in Escherichia coli and was isolated as two colored species (green and red). The green species is a monomer of the sensor domain with a five-coordinated high-spin heme and the red species is probably a noncovalent dimer of the sensor domain which might have an uncharacterized ligand bound to the dimer. The UV-VIS spectrum of the green species indicates that it has a c'-type heme, but its structure is predicted to be homologous to CitA, a periplasmic PAS domain that does not contain heme. The GSU0303 sensor domain represents a previously unreported family of PAS-type periplasmic sensor domains that contain c-type hemes; these proteins could be part of an important mechanism for sensing redox potential or small ligands in the periplasm. Homologs to the sensor domains we identified in G. sulfurreducens are observed in various bacteria although ...Continue Reading

References

Mar 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M A CusanovichM D Kamen
Jan 1, 1993·Annual Review of Microbiology·D R Lovley
Aug 1, 1995·Protein Science : a Publication of the Protein Society·K C KeilerR T Sauer
Feb 2, 1996·The Journal of Biological Chemistry·K C Keiler, R T Sauer
Jul 1, 1996·Protein Science : a Publication of the Protein Society·P M MatiasL Sieker
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Oct 29, 1998·Biochemical and Biophysical Research Communications·E ArslanL Thöny-Meyer
Mar 2, 1999·Journal of Structural Biology·S L Mowbray, M O Sandgren
Aug 19, 1999·Protein Science : a Publication of the Protein Society·R B Kapust, D S Waugh
Jun 22, 2000·Journal of Molecular Biology·L A KelleyM J Sternberg
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Jun 19, 2001·Trends in Biochemical Sciences·A H West, A M Stock
Oct 9, 2001·Bioinformatics·G E Tusnády, I Simon
Sep 25, 2002·Biochemistry·Anatoly L Mayburd, Richard J Kassner
Dec 3, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rinku Jain, Michael K Chan
Mar 27, 2003·Acta Crystallographica. Section D, Biological Crystallography·David AragãoMaria Arménia Carrondo
Apr 4, 2003·Analytical Biochemistry·Robert FeissnerRobert G Kranz
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Hendrik Szurmant, George W Ordal
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Nov 3, 2004·Advances in Microbial Physiology·Derek R LovleyKelly P Nevin
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·George H Wadhams, Judith P Armitage
Dec 16, 2004·Journal of Inorganic Biochemistry·Marie-Alda Gilles-Gonzalez, Gonzalo Gonzalez
Nov 16, 2005·Biochemistry·Shiro YoshiokaShigetoshi Aono
Mar 29, 2006·Journal of Bioinformatics and Computational Biology·Emily W XuDaniel G Brown

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Citations

Mar 11, 2008·Journal of Bacteriology·Robert L KerbyGary P Roberts
Dec 17, 2009·Journal of Bacteriology·Changsoo ChangMarianne Schiffer
Jun 15, 2011·Annual Review of Microbiology·Jonathan T Henry, Sean Crosson
Dec 25, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lucia BanciShenlin Wang
Jul 29, 2008·Protein Expression and Purification·Yuri Y LonderFrank R Collart
Jul 22, 2008·Biochimica Et Biophysica Acta·Yan-Huai R DingTünde Mester
Nov 26, 2013·Journal of Inorganic Biochemistry·Bruno M FonsecaRicardo O Louro
Nov 22, 2008·Biochemical Society Transactions·Stephen Spiro
Aug 28, 2021·International Journal of Molecular Sciences·Marta A Silva, Carlos A Salgueiro

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