Characterization of a carotenoid-hyperproducing yeast mutant isolated by low-dose gamma irradiation

International Journal of Food Microbiology
Namkyu SunKyung Bin Song

Abstract

To isolate a carotenoid-hyperproducing yeast, Phaffia rhodozyma was treated by low-dose gamma irradiation below 10 kGy. Through repeated rounds of gamma irradiation and visual screening, a mutant 3A4-8 was isolated. It produced 3.3 mg of carotenoid per gram of yeast, 50% higher carotenoid content than that of the unirradiated strain. Glucose and peptone were the most suitable carbon and nitrogen sources for production of carotenoid based on the growth experiment of the mutant under various carbon and nitrogen sources. This result suggests that low-dose gamma irradiation could be used as a means of mutagenesis for isolation of a carotenoid-hyperproducing strain of P. rhodozyma because only the carotenoid-hyperproducing yeast survives gamma irradiation by scavenging oxygen radicals generated by radiolysis of water.

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Citations

Nov 4, 2010·Applied Microbiology and Biotechnology·Isabell SchmidtJens Schrader
Nov 5, 2008·Journal of Industrial Microbiology & Biotechnology·Ginka I Frengova, Dora M Beshkova
Mar 17, 2010·Journal of Industrial Microbiology & Biotechnology·Lili MiaoYing Li
Oct 6, 2009·Applied and Environmental Microbiology·Ken UkibeHiroshi Takagi
Jan 22, 2014·Microbial Cell Factories·Luis Carlos Mata-GómezCristóbal Noé Aguilar
Aug 17, 2010·Applied Microbiology and Biotechnology·Marta Rodríguez-SáizJosé Luis Barredo
Aug 23, 2011·Canadian Journal of Microbiology·Naimeh NajafiShokoofeh Golkhoo
Sep 16, 2008·Journal of Basic Microbiology·Marcelo BaezaVíctor Cifuentes
Feb 3, 2005·Yeast
Sep 15, 2021·Applied Biochemistry and Biotechnology·Xuejiao TianJihong Chen

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