PMID: 9551184Jan 1, 1997Paper

Characterization of a cDNA encoding a novel band 4.1-like protein in zebrafish

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
G M Kelly, B Reversade

Abstract

Membrane skeleton protein 4.1 and other members of a family of proteins that link the cytoskeleton to the plasma membrane may play an integral role in cell communication during development. The polymerase chain reaction and degenerate oligodeoxynucleotide primers to consensus sequences in the putative membrane-binding domain of the protein 4.1 superfamily were used to isolate cDNAs encoding members of the zebrafish protein 4.1 family. Zebrafish stage- and tissue-specific first strand cDNA was used in the PCR. After the reaction, amplicons of the predicted size were sequenced to confirm their relationship to the protein 4.1 superfamily. One cDNA, with a high degree of similarity to a mouse novel band 4.1-like cDNA, was used to probe a zebrafish adult brain library. A 2.4-kb cDNA was isolated and found to encode a 619 amino acid polypeptide homologous to mouse novel band 4.1-like protein 4. Zebrafish nbl4 mRNA is maternally supplied and is expressed throughout embryogenesis. In adults, nbl4 is found in the ovary, eye, heart, and brain, but not in gut or skeletal muscle. When synthetic nbl4 mRNA is translated in vitro it binds calmodulin in a calcium-dependent manner. These data indicate that zebrafish nbl4 is a maternal transcrip...Continue Reading

Citations

Sep 14, 2000·Genome Research·W B BarbazukS L Johnson
May 11, 2011·Development Genes and Evolution·Yanchun GuoMichael Kühl
Jul 16, 2009·Gene Expression Patterns : GEP·Chuan-Yang FuHuai-Jen Tsai
Dec 4, 2003·Developmental Biology·Michelle N KnowltonGregory M Kelly
May 30, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G M KellyS Mangos

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