Characterization of a cDNA encoding the thylakoidal processing peptidase from Arabidopsis thaliana. Implications for the origin and catalytic mechanism of the enzyme.

The Journal of Biological Chemistry
B K ChaalC J Howe

Abstract

We have identified and sequenced a cDNA containing a complete open reading frame for a putative 340-amino acid precursor of the thylakoidal processing peptidase from Arabidopsis thaliana. The predicted amino acid sequence of the protein includes regions highly conserved among Type I leader peptidases and indicates that the enzyme uses a serine-lysine catalytic dyad mechanism. Phylogenetic analysis indicated a common ancestry of the enzyme with those from oxygenic photosynthetic prokaryotes, suggesting that the cDNA encoded the chloroplast enzyme. The catalytic domain was overexpressed in Escherichia coli, generating a product capable of cleaving the thylakoid-transfer domain from a chloroplast protein. Antibodies to the overexpressed polypeptide cross-reacted with a 30-kDa thylakoid membrane protein.

References

Nov 1, 1992·Trends in Biochemical Sciences·R E Dalbey, G Von Heijne
Mar 1, 1992·The Biochemical Journal·M T BlackA E Allsop
Apr 1, 1989·European Journal of Biochemistry·G von HeijneR G Herrmann
Jan 1, 1988·Annual Review of Genetics·J Felsenstein
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 1, 1996·Biochemical Society Transactions·C J HoweJ C Packer
Jan 1, 1997·Trends in Biochemical Sciences·M Paetzel, R E Dalbey
Apr 1, 1998·Bioinformatics·D H Huson
Jun 1, 1995·Trends in Cell Biology·J C Gray, P E Row

❮ Previous
Next ❯

Citations

Aug 5, 2000·Pharmacology & Therapeutics·M PaetzelN C Strynadka
Dec 23, 1999·Current Opinion in Plant Biology·G I McFadden
Apr 24, 1999·The Plant Cell·K Keegstra, K Cline
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Ross E DalbeyJan Maarten van Dijl
May 4, 2006·Annual Review of Plant Biology·Wataru Sakamoto
Aug 10, 2006·BMC Genomics·Lokesh P Tripathi, R Sowdhamini
Nov 9, 2005·The Journal of Cell Biology·Kentaro InoueMasaru Ohme-Takagi
Sep 4, 2014·The Plant Journal : for Cell and Molecular Biology·Takafumi MidorikawaKentaro Inoue
Jun 28, 2013·The Plant Journal : for Cell and Molecular Biology·Takafumi Midorikawa, Kentaro Inoue
Jun 8, 2011·Biochimica Et Biophysica Acta·Wei ChiLixin Zhang
Dec 21, 2010·Biochimica Et Biophysica Acta·Paul Dominic B OlinaresKlaas J van Wijk
Dec 8, 2010·Plant Signaling & Behavior·Joshua K EndowKentaro Inoue
Aug 12, 2000·Journal of Experimental Botany·C RobinsonW Edwards
Oct 9, 2002·The Journal of Biological Chemistry·Stéphane MirasNorbert Rolland
Dec 12, 2002·Chemical Reviews·Mark PaetzelRoss E Dalbey

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.