Characterization of a chitinase (Chit62) from Serratia marcescens B4A and its efficacy as a bioshield against plant fungal pathogens

Biochemical Genetics
S BabashpourK Haghbeen

Abstract

Chitinases have been suggested to be involved in pathogen-antagonist interaction during biological control progress of plant pathogenic fungi. Here, a recombinant bacterial chitinase originally from Serratia marcescens B4A was produced, purified, and assayed biochemically to ascertain the activity and determine the kinetics parameters. Active enzyme was used to determine its biocontrol features against fungal phytopathogens. The results demonstrated that the optimum pH and temperature for the enzyme activity were 6.0 and 55 °C, respectively. The K(m) and V(max) values were 3.30 mg ml(-1) and 0.92 units, respectively. The recombinant chitinase was demonstrated to be highly active in controlling fungal pathogens.

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Citations

Sep 10, 2013·Canadian Journal of Microbiology·Lauren M Petersen, Louis S Tisa
Aug 17, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Nasrin AliabadiKamahldin Haghbeen
Apr 11, 2017·PloS One·Fatemeh Zebardast RoodiKamahldin Haghbeen
Jul 15, 2015·Applied and Environmental Microbiology·Kun JiangJun Cai
Jan 1, 2016·Pakistan Journal of Biological Sciences : PJBS·Siti Nur AisyahJamsari Jamsari
Jul 19, 2019·International Journal of Molecular Sciences·Samantha Sarai Navarro-GonzálezRamón Suárez-Rodríguez

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