Characterization of a cinnamoyl-CoA reductase 1 (CCR1) mutant in maize: effects on lignification, fibre development, and global gene expression

Journal of Experimental Botany
Barek TamasloukhtMagalie Pichon

Abstract

Cinnamoyl-CoA reductase (CCR), which catalyses the first committed step of the lignin-specific branch of monolignol biosynthesis, has been extensively characterized in dicot species, but few data are available in monocots. By screening a Mu insertional mutant collection in maize, a mutant in the CCR1 gene was isolated named Zmccr1(-). In this mutant, CCR1 gene expression is reduced to 31% of the residual wild-type level. Zmccr1(-) exhibited enhanced digestibility without compromising plant growth and development. Lignin analysis revealed a slight decrease in lignin content and significant changes in lignin structure. p-Hydroxyphenyl units were strongly decreased and the syringyl/guaiacyl ratio was slightly increased. At the cellular level, alterations in lignin deposition were mainly observed in the walls of the sclerenchymatic fibre cells surrounding the vascular bundles. These cell walls showed little to no staining with phloroglucinol. These histochemical changes were accompanied by an increase in sclerenchyma surface area and an alteration in cell shape. In keeping with this cell type-specific phenotype, transcriptomics performed at an early stage of plant development revealed the down-regulation of genes specifically assoc...Continue Reading

References

Apr 28, 2000·Nature·S J LiljegrenM F Yanofsky
Jun 8, 2001·The Plant Journal : for Cell and Molecular Biology·L JonesS R Turner
Jan 20, 2004·Plant Physiology·Mireille CabanéCatherine Lapierre
Nov 24, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N O I CoganJ W Forster
Jun 23, 2005·The Biochemical Journal·Anna KärkönenStephen C Fry
Jul 12, 2005·Biological Chemistry·Qing-Hu Ma, Bin Tian
Dec 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tsutomu KawasakiKo Shimamoto
Jan 20, 2006·The Journal of Biological Chemistry·John RalphRichard A Dixon
Nov 10, 2006·The Plant Journal : for Cell and Molecular Biology·Dhrubojyoti D LaskarNorman G Lewis
Nov 14, 2006·Nucleic Acids Research·Tanya BarrettRon Edgar
Oct 18, 2007·Nucleic Acids Research·Séverine GagnotVéronique Brunaud
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Heather D ColemanShawn D Mansfield
Mar 18, 2008·Plant Physiology·Ricardo A Chávez MontesDeborah Goffner
Aug 30, 2008·The Plant Cell·Ayako NakashimaKo Shimamoto
May 7, 2010·The Plant Journal : for Cell and Molecular Biology·Mu ZhangYihua Zhou
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Rui ZhouRichard A Dixon

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Citations

Jan 30, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Audrey CourtialYves Barrière
Jan 1, 2014·Proceedings of the National Academy of Sciences of the United States of America·Rebecca Van AckerWout Boerjan
Nov 15, 2015·Plant Science : an International Journal of Experimental Plant Biology·Yves BarrièreJacqueline Grima-Pettenati
Jan 23, 2016·Molecular Breeding : New Strategies in Plant Improvement·Claire Lessa Alvim KameiLuisa M Trindade
Jan 31, 2012·Plant Science : an International Journal of Experimental Plant Biology·John GrayErich Grotewold
Nov 30, 2013·The Plant Journal : for Cell and Molecular Biology·Ho Man TangPatrick S Schnable
Feb 17, 2015·The New Phytologist·Victor CarochaJacqueline Grima-Pettenati
Dec 17, 2014·Journal of Plant Physiology·Francisco Fernández-PérezEsther Novo-Uzal
Feb 26, 2014·Critical Reviews in Biochemistry and Molecular Biology·Zhangying Hao, Debra Mohnen
Oct 28, 2014·Frontiers in Plant Science·Su-Ying YehWilfried Schwab
Oct 18, 2016·Frontiers in Plant Science·Anne-Laure Chateigner-BoutinLuc Saulnier
Oct 21, 2016·Applied Biochemistry and Biotechnology·Lakshmi KasirajanSelvi Athiappan
Jan 10, 2018·Frontiers in Plant Science·Hye Lin ParkMan-Ho Cho
Apr 1, 2019·Journal of Dairy Science·A T AdesoganD Vyas
Apr 15, 2020·The New Phytologist·Joshua H CoomeySamuel P Hazen
Aug 25, 2015·Journal of Integrative Plant Biology·Jinmi YoonGynheung An
Mar 4, 2020·Molecular Genetics and Genomics : MGG·Douglas Jardim-MessederGilberto Sachetto-Martins
Mar 27, 2019·Metabolomics : Official Journal of the Metabolomic Society·Jessica M WedowElizabeth A Ainsworth
Nov 10, 2017·Plant Methods·David LeglandMatthieu Reymond
Jan 7, 2021·Functional & Integrative Genomics·Douglas Jardim-MessederGilberto Sachetto-Martins
Jan 14, 2020·Plant Science : an International Journal of Experimental Plant Biology·Lina Gomez-CanoJohn Gray
Mar 20, 2021·Plant Molecular Biology·K Hodgson-KratkyR J Henry

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Datasets Mentioned

BETA
GSE
11531

Methods Mentioned

BETA
transgenic
PCR
GTPase

Software Mentioned

Image Pro
Image PRO - Plus

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