PMID: 377227Apr 1, 1979Paper

Characterization of a cloned ribosomal fragment from mouse which contains the 18S coding region and adjacent spacer sequences

Nucleic Acids Research
I GrummtI Scholz

Abstract

The large EcoRI fragment of mouse ribosomal genes containing parts of the non-transcribed spacer, the external transcribed spacer located at the 5' end of the precursor molecule and about two thirds of the 18S sequence has been cloned in bacteriophage lambda gtWES. A physical map of the DNA was constructed by cleavage with several restriction endonucleases and hybridization of the restriction fragments of the recombinant DNA with labelled 18S and 45S rRNA. The orientation of the inserted fragment as well as the length of the 18S sequence was determined by electron microscopy of R-loop containing molecules. The absence of hybridization of the cloned fragment to other fragments in the genome shows that the non-transcribed spacer does not have a significant length of sequences in common with other sequences in the genome.

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Citations

Jan 1, 1980·Molecular & General Genetics : MGG·I Grummt, H J Gross
Dec 1, 1982·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R Appels, J Dvořák
Jan 1, 1981·Molecular & General Genetics : MGG·M FukeH Busch
Jan 1, 1982·Gene·L I RothblumB Cassidy
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I Grummt
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R ReichelB J Benecke
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G BoucheF Amalric
Jan 1, 1988·The Journal of Cell Biology·C A Haas, L J DeGennaro
May 1, 1995·The Journal of Cell Biology·D Weisenberger, U Scheer
Sep 24, 1983·Nucleic Acids Research·D M ChikaraishiC A Harrington
Dec 11, 1979·Nucleic Acids Research·W L Gerlach, J R Bedbrook
Oct 10, 1981·Nucleic Acids Research·L H BowmanD Schlessinger
Jun 17, 2010·Genes & Development·Courtney H CoschiFrederick A Dick
Sep 16, 2006·Cytogenetic and Genome Research·R WimmerI Hansmann
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Oct 1, 1989·Analytical Biochemistry·B LomholtS Frederiksen
Oct 1, 1991·Kidney International·E Schulze-LohoffR B Sterzel

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