PMID: 2120708Oct 1, 1990Paper

Characterization of a complete immunoglobulin heavy-chain variable region germ-line gene of rainbow trout

Proceedings of the National Academy of Sciences of the United States of America
T MatsunagaV Törmänen

Abstract

A germ-line heavy-chain variable region (VH) gene (RTVH431) has been isolated from a rainbow trout (Salmo gairdneri) and characterized by complete nucleotide sequencing. It is characteristic of VH, as shown by the conserved octamer and TATA motif in the 5' region, the heptamernonamer recombination signal sequence in the 3' region, and the 18-amino-acid-long hydrophobic leader interrupted by an intron. The 98-amino-acid-long VH coding region has 50-70% nucleotide sequence homology and 40-60% amino acid sequence homology with VHS of various vertebrate species. We have also found unique or species-specific amino acid residues in the VHS of rainbow trout, amphibia (Xenopus), reptile (Caiman), and shark (Heterodontus) in our sequence analyses. The RTVH431 has an unusual amino acid in the conserved 34th position in complementarity-determining region 1 of VH. Southern hybridization results suggest the presence of a large gene family related to RTVH431 in the trout genome. The complex evolution of antibody V genes is discussed.

References

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Citations

Jan 1, 1994·Immunogenetics·C Michard-VanhéeL Pilström
Jan 1, 1995·Developmental and Comparative Immunology·G W Warr
May 1, 1996·Research in Immunology·E Andersson, T Matsunaga
Apr 15, 1994·Annals of the New York Academy of Sciences·T MatsunagaE Andersson
Oct 4, 2005·Veterinary Immunology and Immunopathology·Chao-An ChengChi-Yao Chang
Aug 9, 2005·Developmental and Comparative Immunology·Stein Tore Solem, Jørgen Stenvik
Oct 1, 1991·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·G W WarrM R Wilson

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