Characterization of a cytochrome P-450 dependent monoterpene hydroxylase from the higher plant Vinca rosea

K M MadyasthaC J Coscia


A monooxygenase isolated from 5-day old etiolated Vinca rosea seedlings was shown to catalyze the hydroxylation of the monoterpene alcohols, geraniol and nerol, to their corresponding 10-hydroxy derivatives. Hydroxylase activity was inpendent upon NADPH (neither NADH nor combination of NADH, NADP+ and ATP served as substitutes) and O2. Geraniol hydroxylation was enhanced by dithiothreitol (monothiols were less effective) and inhibited by phospholipases, thiol reagents, metyrapone, and cytochrome c, as well as other inhibitors of cytochrome P-450 systems. Geraniol was hydroxylated at a faster rate than nerol, but the alcohols possessed similar apparent Km values. The membrane-bound hydroxylase was solubilized by treatment with sodium cholate, Renex-30, or Lubrol-WX. Cholate-treated enzyme was resolved by DEAE-cellulose chromatography and reconstitution of the hydroxylase was effected utilizing different fractions containing cytochrome P-450, a NADPH-cytochrome c reductase, and lipid.


Jan 1, 1975·Annual Review of Biochemistry·I C GunsalusS G Sligar
Aug 21, 1973·Biochemical and Biophysical Research Communications·T D Meehan, C J Coscia
Sep 1, 1969·Archives of Biochemistry and Biophysics·P J Murphy, C A West
Apr 1, 1972·Chemical Reviews·D V BanthorpeM J Francis
Sep 16, 1974·Biochimica Et Biophysica Acta·A Y Lu, W Levin
Apr 4, 1969·Analytical Biochemistry·J L Gaylor, C V Delwiche
Jun 1, 1969·Analytical Biochemistry·V H Potty


Feb 1, 1988·Mutation Research·J M Gentile, M J Plewa
Feb 1, 1986·Mutation Research·J M GentileM J Plewa
Jun 1, 1987·Biochimie·J L Riviere, F Cabanne
Dec 1, 1994·Phytochemistry·G P BolwellA Zimmerlin
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·W M Chou, T M Kutchan
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M S ShetR W Estabrook
Nov 1, 1992·Plant Physiology·M Simontacchi, S Puntarulo
Jun 1, 1984·Plant Physiology·C M Chen, S M Leisner
Apr 1, 1992·Plant Physiology·D L HallahanR M Wallsgrove
Aug 12, 2010·Bioscience, Biotechnology, and Biochemistry·Junfeng WangFengning Xiang
Jan 1, 1995·Drug Metabolism and Drug Interactions·R E ChristoffersenK Bozak
Jan 1, 1995·Drug Metabolism and Drug Interactions·S LupienR Croteau
Feb 1, 1993·Phytochemistry·T M Kutchan
May 1, 1984·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A Chadha, K M Madyastha
Jan 1, 1981·Drug Metabolism Reviews·D R Dohn, R I Krieger
Apr 29, 1986·Biochemical and Biophysical Research Communications·K M Madyastha, N Krishnamachary
Jun 1, 1978·Archives of Biochemistry and Biophysics·C L Soliday, P E Kolattukudy
Oct 15, 1982·Biochemical and Biophysical Research Communications·A Chadha, K M Madyastha
Jan 22, 1998·Archives of Biochemistry and Biophysics·A RoscoT M Kutchan
May 21, 2016·Frontiers in Plant Science·Tina IlcD Werck-Reichhart
Dec 26, 1978·Biochemistry·H J Licht, C J Coscia

Related Concepts

Carbon Monoxide
Cytochrome P-450 Oxygenase
Synthetic Detergent
Reduced Glutathione
Hydrogen-Ion Concentration
Mixed Function Oxygenases

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.