Characterization of a cytosolic malate dehydrogenase cDNA which encodes an isozyme toward oxaloacetate reduction in wheat

Biochimie
Yu Ding, Qing-Hu Ma

Abstract

Malate dehydrogenase (MDH), which is ubiquitous in nature, catalyzes the interconversion of oxaloacetate and malate. Higher plants contain multiple forms of MDH that differ in co-enzyme specificity, subcellular localization and physiological function. Cytosolic NAD-dependent MDH (cyMDH) is one class of MDH that has not been extensively characterized in plants. Here we report the cloning of a cDNA from wheat by RT-PCR and cDNA library screening, which is designated as TaMDH. Sequence analysis indicated that TaMDH exhibits a highly similarity to other plant cyMDHs. Immunological analysis confirmed that TaMDH encoded a cytosolic NAD-dependent MDH. The secondary and three-dimensional structures of TaMDH were analyzed by molecular modeling. DNA gel-blot analyses demonstrated that TaMDH gene exists as two copies in the wheat genome. RNA and protein gel-blot hybridization indicated that both TaMDH mRNA and protein were constitutively expressed in vegetative tissues of wheat, with slightly lower levels in roots than in leaves and stems. In silico analysis indicated that TaMDH was also expressed in various reproductive tissues and tissues under many different stress conditions. Kinetic analysis of bacterially expressed and purified prot...Continue Reading

References

May 1, 1975·Canadian Journal of Biochemistry·A J Legris, C S Tsai
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Apr 2, 1974·Archives of Biochemistry and Biophysics·N S Yang, J G Scandalios
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J J BirktoftL J Banaszak
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Nov 1, 1996·European Journal of Biochemistry·M G Claros, P Vincens
Oct 17, 1998·The Journal of Biological Chemistry·M BerkemeyerO Ocheretina
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch

❮ Previous
Next ❯

Citations

Nov 11, 2008·Retina·Edward ChaumKenneth W Tobin
Jan 18, 2011·Plant Physiology and Biochemistry : PPB·Yu-Xin YaoYu-Jin Hao
Sep 19, 2015·The Arabidopsis Book·Veronica G Maurino, Martin K M Engqvist
Dec 30, 2014·Frontiers in Plant Science·Setsuko KomatsuZahed Hossain
Nov 2, 2006·DNA Sequence : the Journal of DNA Sequencing and Mapping·Bin TianQing-Hu Ma
Dec 21, 2013·Molecular Biology Reports·Myeong Won OhSun-Hee Woo
Oct 17, 2019·Plant Molecular Biology·Xi WuShuqing Cao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.