Characterization of a detergent-resistant surface lamina in cultured human fibroblasts

Experimental Cell Research
V P LehtoI Virtanen

Abstract

Treatment of cultured human fibroblasts with 0.5% Triton X-100 produces substratum-anchored cytoskeletal preparations consisting of cytoplasmic filaments, nucleus and a plasma membrane-derived surface lamina. The lamina was visualized in fluorescence microscopy with fluorochrome-coupled wheat germ agglutinin (WGA) as a lace-like structure, extending throughout the cell domain. It displayed a different organization at the ventral and dorsal surfaces of the cell, partially coaligning with bundles of actin and myosin filaments at the dorsal cell surface. At the ventral surface vinculin patches appeared to be included in the surface lamina. Polyacrylamide gel electrophoresis, combined with lectin reactivity studies and lectin affinity chromatography, revealed a 140 kD sialoglycoprotein as the major glycoprotein component of the surface lamina.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 25, 1978·Nature·J Flanagan, G L Koch
Jan 1, 1976·Journal of Supramolecular Structure·S BrownJ A Spudich
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J F Ash, S J Singer
Apr 1, 1980·Experimental Cell Research·R A BadleyD A Rees
Apr 1, 1981·Cell Biology International Reports·V P LehtoI Virtanen
Aug 14, 1980·Biochemical and Biophysical Research Communications·V P LehtoI Virtanen

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