Characterization of a DNA damage-inducible membrane protein kinase from Deinococcus radiodurans and its role in bacterial radioresistance and DNA strand break repair

Molecular Microbiology
Yogendra S Rajpurohit, Hari S Misra

Abstract

Deinococcus radiodurans mutant lacking pyrroloquinoline-quinone (PQQ) synthesis shows sensitivity to γ-rays and impairment of DNA double strand break repair. The genome of this bacterium encodes five putative proteins having multiple PQQ binding motifs. The deletion mutants of corresponding genes were generated, and their response to DNA damage was monitored. Only the Δdr2518 mutant exhibited higher sensitivity to DNA damage. Survival of these cells decreased by 3-log cycle both at 6 kGy γ-rays and 1200 Jm(-2) UV (254 nm) radiation, and 2.5-log cycle upon 14 days desiccation at 5% humidity. The Δdr2518 mutant showed complete inhibition of DSB repair until 24 h PIR and disappearance of a few phosphoproteins. The Δdr2518pqqE:cat double mutant showed γ-ray sensitivity similar to Δdr2518 indicating functional interaction of these genes in D. radiodurans. DR2518 contains a eukaryotic type Ser/Thr kinase domain and structural topology suggesting stress responsive transmembrane protein. Its autokinase activity in solution was stimulated by nearly threefold with PQQ and twofold with linear DNA, but not with circular plasmid DNA. More than 15-fold increase in dr2518 transcription and several-fold enhanced in vivo phosphorylation of DR25...Continue Reading

References

Mar 5, 1990·Journal of Molecular Biology·M Sassanfar, J W Roberts
Jul 15, 1983·Journal of Molecular Biology·J W Little
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J W LittleD W Mount
Jan 1, 1995·Experimental Cell Research·K Mishra, V K Parnaik
Jun 4, 1993·Cell·J S Parkinson
Jan 16, 1999·Advances in Microbial Physiology·P M Goodwin, C Anthony
Nov 24, 1999·Molecular Microbiology·R DuttaM Inouye
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A Kuzminov
Mar 14, 2000·Current Biology : CB·J R Battista
Apr 29, 2000·Trends in Microbiology·Y Av-Gay, M Everett
Dec 2, 2000·Nature·B B Zhou, S J Elledge
Apr 28, 2001·Journal of Bacteriology·R MeimaM E Lidstrom
Apr 23, 2002·Molecular Microbiology·Pavel P Khil, R Daniel Camerini-Otero
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
Sep 21, 2002·Molecular Genetics and Genomics : MGG·C Bonacossa de AlmeidaA Bailone
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yongqing LiuMichael J Daly
Apr 25, 2003·Nature·Takaoki Kasahara, Tadafumi Kato
Mar 17, 2004·Free Radical Biology & Medicine·Robert J WilliamsCatherine Rice-Evans
Jun 11, 2004·Annual Review of Biochemistry·Aziz SancarStuart Linn
Jul 14, 2004·Biochemical and Biophysical Research Communications·Bhakti JoshiShree Kumar Apte
Dec 8, 2004·FEBS Letters·Hari S MisraShree K Apte
Jan 27, 2005·Journal of Biochemistry·Duohong ShengYuejin Hua
Nov 2, 2005·Nature Reviews. Microbiology·Michael M Cox, John R Battista
Mar 22, 2006·Nucleic Acids Research·Ivan MijakovicDusica Vujaklija
Jul 28, 2006·Cold Spring Harbor Symposia on Quantitative Biology·R Kitagawa, M B Kastan
Sep 29, 2006·Nature·Ksenija ZahradkaMiroslav Radman

❮ Previous
Next ❯

Citations

May 15, 2012·Journal of Biosciences·H S MisraN P Khairnar
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Dea Slade, Miroslav Radman
Apr 3, 2016·Mutation Research. Reviews in Mutation Research·Athanasia PavlopoulouAlexandros G Georgakilas
Jan 29, 2019·Journal of Biomolecular Structure & Dynamics·Dhirendra Kumar SharmaYogendra Singh Rajpurohit
Sep 30, 2020·Journal of Biomolecular Structure & Dynamics·Dhirendra K SharmaYogendra S Rajpurohit
Jun 4, 2016·The Journal of Biological Chemistry·Yogendra S RajpurohitHari S Misra
Sep 14, 2017·Antioxidants & Redox Signaling·Subhash C BihaniVinay Kumar
Aug 7, 2019·International Journal of Systematic and Evolutionary Microbiology·Xin-Peng WangDa-Shuai Mu
Jun 21, 2020·World Journal of Microbiology & Biotechnology·Burak AlaylarNaveen Kumar Arora
May 8, 2021·Frontiers in Cell and Developmental Biology·Yogendra Singh RajpurohitHari S Misra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.