Characterization of a G-protein activator in the neuroblastoma-glioma cell hybrid NG108-15.

The Journal of Biological Chemistry
M SatoS M Lanier

Abstract

Purified bovine brain G-protein was used in a solution phase assay to identify membrane-associated proteins that influenced the activation of heterotrimeric G-proteins. Detergent-solubilized membrane extracts from the neuroblastoma-glioma cell hybrid NG108-15, but not the parent C6B4 glioma cell line, increased [35S]GTPgammaS binding to purified G-protein by approximately 460%. The G-protein activator was heat-sensitive, and the magnitude of its action was related to the amount of extract protein. The biophysical and biochemical properties of the G-protein activator were determined using DEAE ion exchange chromatography, gel filtration, and a lectin affinity matrix. In the presence of added GDP (1 microM), the enriched G-protein activator increased the initial rate of [35S]GTPgammaS binding to brain G-protein by up to 4-fold. In the absence of added GDP, the G-protein activator elicited an initial burst in [35S]GTPgammaS binding to brain G-protein within the first 30 s, after which the rate of nucleotide binding to G-protein was similar in the absence or presence of the G-protein activator. The stimulation of nucleotide binding to brain G-protein by the activator was also observed after resolution of Galpha from Gbetagamma. The...Continue Reading

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Citations

Jul 5, 2005·Nature Immunology·Yuya TerashimaKouji Matsushima
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rama KatariaArjan Kortholt
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·Motohiko SatoStephen M Lanier
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Motohiko SatoStephen M Lanier
Jul 26, 2005·Reviews of Physiology, Biochemistry and Pharmacology·M J Cismowski, S M Lanier
Jun 9, 2009·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Motohiko Sato, Yoshihiro Ishikawa
Jan 24, 2007·Pharmacology & Therapeutics·Joe B BlumerStephen M Lanier
Apr 20, 2006·Seminars in Cell & Developmental Biology·Mary J Cismowski
Aug 1, 1997·Biochemical Pharmacology·J D Hildebrandt
Jun 3, 2006·Developmental Cell·Dandan ShanXin-Yun Huang
Aug 16, 2016·Journal of Molecular Biology·Viktoriya SyrovatkinaXin-Yun Huang
Nov 12, 2016·American Journal of Physiology. Cell Physiology·Abdullah Al MamunMotohiko Sato
Sep 3, 1999·Nature Biotechnology·R M Frederickson
Apr 29, 1998·The Journal of Biological Chemistry·G WuS M Lanier
Aug 27, 1999·Molecular Pharmacology·Q Yang, S M Lanier
Nov 24, 1999·The Journal of Biological Chemistry·A TakesonoS M Lanier
Nov 12, 2002·The Journal of Biological Chemistry·Catalina RibasStephen M Lanier
Jan 2, 2003·The Journal of Biological Chemistry·Gregory G TallAlfred G Gilman

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