Characterization of a galectin-like activity from the parasitic nematode, Haemonchus contortus, which modulates ovine eosinophil migration in vitro

Veterinary Immunology and Immunopathology
Darryl G TurnerDouglas G Jones

Abstract

The development of eosinophilia is a characteristic feature of helminth infection, although the exact nature of the interaction between eosinophils and parasites remains to be fully defined. Previously, it has been reported that Haemonchus contortus and other nematodes produce eosinophil-specific chemoattractants. This paper describes studies aimed at isolating and identifying the factor(s) responsible. Initial studies showed that soluble extracts of infective larvae (L3) of H. contortus provoked a chemokinetic, rather than chemotactic, response in ovine bone marrow eosinophils in vitro. This activity was inhibited by lactose to a markedly greater extent than sucrose suggesting a galectin-like identity. Lactose affinity chromatography of soluble H. contortus extracts resulted in the isolation a specific bound fraction which retained biological activity. SDS-PAGE gel electrophoresis indicated a single Coomassie-stained band at between 31 and 41kDa. Subsequent, mass spectrometric analysis confirmed that the bound fraction contained a mixture of nematode galectins. The results confirm that H. contortus larvae produce several galectin-like proteins, at least one of which demonstrates eosinophil chemokinetic activity in vitro. The p...Continue Reading

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Citations

Apr 9, 2009·PLoS Neglected Tropical Diseases·Sasisekhar BennuruThomas B Nutman
May 16, 2009·Nature Reviews. Microbiology·Gerardo R Vasta
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sasisekhar BennuruThomas B Nutman
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Aug 30, 2008·Molecular & Cellular Proteomics : MCP·Jason MulvennaJeffrey J Gorman
Nov 4, 2009·The Korean Journal of Parasitology·Myeong Heon ShinDuk-Young Min
Feb 23, 2019·Parasitology Research·Jinglong ChenXiaojing Yang
Oct 9, 2014·Parasite : Journal De La Société Française De Parasitologie·Tom N McNeilly, Alasdair J Nisbet
Mar 25, 2021·PLoS Neglected Tropical Diseases·Katalina CruzMaría Trelis
Jul 31, 2018·Veterinary Parasitology·Márcia B da SilvaNeuza M Alcantara-Neves

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