Characterization of a Gene Coding for the Complement System Component FB from Loxosceles laeta Spider Venom Glands

PloS One
Daniela Tiemi MyamotoDenise V Tambourgi

Abstract

The human complement system is composed of more than 30 proteins and many of these have conserved domains that allow tracing the phylogenetic evolution. The complement system seems to be initiated with the appearance of C3 and factor B (FB), the only components found in some protostomes and cnidarians, suggesting that the alternative pathway is the most ancient. Here, we present the characterization of an arachnid homologue of the human complement component FB from the spider Loxosceles laeta. This homologue, named Lox-FB, was identified from a total RNA L. laeta spider venom gland library and was amplified using RACE-PCR techniques and specific primers. Analysis of the deduced amino acid sequence and the domain structure showed significant similarity to the vertebrate and invertebrate FB/C2 family proteins. Lox-FB has a classical domain organization composed of a control complement protein domain (CCP), a von Willebrand Factor domain (vWFA), and a serine protease domain (SP). The amino acids involved in Mg2+ metal ion dependent adhesion site (MIDAS) found in the vWFA domain in the vertebrate C2/FB proteins are well conserved; however, the classic catalytic triad present in the serine protease domain is not conserved in Lox-FB....Continue Reading

References

Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Dec 16, 1998·Science·UNKNOWN C. elegans Sequencing Consortium
Jan 23, 1999·Immunological Reviews·J O Sunyer, J D Lambris
Mar 25, 2000·Science·M D AdamsJ C Venter
Oct 17, 2001·Developmental and Comparative Immunology·I K ZarkadisJ D Lambris
Dec 24, 2004·The EMBO Journal·Yong ZhuJeak Ling Ding
Jul 9, 2005·Cellular and Molecular Life Sciences : CMLS·L Polgár
Aug 10, 2006·Immunogenetics·Masaru Nonaka, Ayuko Kimura
Feb 21, 2007·Nature Structural & Molecular Biology·Fin J MilderPiet Gros
Feb 9, 2008·Cellular and Molecular Life Sciences : CMLS·M J Page, E Di Cera
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shigeru ArikiShun-ichiro Kawabata
Dec 17, 2008·Fish & Shellfish Immunology·M Prado-AlvarezA Figueras
Jan 10, 2009·Nature Protocols·Lorenza BordoliTorsten Schwede
Jun 26, 2012·Fish & Shellfish Immunology·Lei ZhongYaqing Chang
Jul 3, 2013·Molecular Immunology·Peter F ZipfelKristian Riesbeck
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
May 7, 2014·Sub-cellular Biochemistry·Masaru Nonaka
Dec 23, 2014·Developmental and Comparative Immunology·Reo Sekiguchi, Masaru Nonaka

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

ExPaSy
Model Workspace
SwissPDBViewer
MEGA
MUSCLE
SWISS
Basic Local Alignment Search Tool ( BLAST )
MUSCLE ( Multiple Sequence Comparison by Log - Expectation )

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