Characterization of a Generalized Transducing Phage of Poly-(gamma)-Glutamic Acid-Producing Bacillus subtilis and Its Application for Analysis of Tn917-LTV1 Insertional Mutants Defective in Poly-(gamma)-Glutamic Acid Production.

Applied and Environmental Microbiology
T Nagai, Yoshifumi Itoh

Abstract

A generalized transducing phage, (phi)BN100, was isolated from a Bacillus subtilis (natto) strain producing poly-(gamma)-glutamic acid ((gamma)PGA). Transduction frequencies for a given marker ranged from 3.8 x 10(sup-8) to 1.6 x 10(sup-6) per phage particle. The genome size of the phage was approximately 42 kb. (phi)BN100 was used successfully to identify bona fide Tn917-LTV1 transpositional mutants defective in (gamma)PGA production.

Citations

Jul 19, 2020·World Journal of Microbiology & Biotechnology·Mengxue Peng, Zhihong Liang
Jul 26, 2008·The Journal of General and Applied Microbiology·Jomkhwan MeerakYasutaka Tahara
Dec 31, 2014·Applied Microbiology and Biotechnology·Mareike Jakobs, Friedhelm Meinhardt
Nov 28, 2018·Biomolecular Concepts·Ekachai ChukeatiroteJuhee Ahn
Sep 8, 2010·Journal of Industrial Microbiology & Biotechnology·Eun Ju KimYoung Nam Lee

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