Characterization of a Genomic Region Required for Production of the Antibiotic Pyoluteorin by the Biological Control Agent Pseudomonas fluorescens Pf-5.

Applied and Environmental Microbiology
J Kraus, J E Loper

Abstract

A 21-kb region required for the biosynthesis of the polyketide antibiotic pyoluteorin by the biological control agent Pseudomonas fluorescens Pf-5 was identified and cloned. Seven previously isolated mutants deficient in pyoluteorin production (Plt(sup-)) had Tn5 insertions spanning the 21-kb region. Sequences flanking Tn5 inserts were cloned from genomic DNA of three Plt(sup-) mutants and used as probes to identify wild-type alleles of the plt loci from a genomic library of Pf-5. Five cosmids containing overlapping regions of genomic DNA hybridized to one or more of the probes. One cosmid, pJEL1938, contained the entire 21-kb region and, when introduced into a Plt(sup-) mutant, partially restored pyoluteorin production. To study the expression of the genes required for pyoluteorin biosynthesis, the transposon Tn3-nice, which contains a promoterless ice nucleation gene (inaZ) and a type I neomycin phosphotransferase gene, was introduced into the genomic plt region of Pf-5. Carbon sources that influenced pyoluteorin production by Pf-5 had parallel effects on ice nucleation activity of Pf-5 containing a genomic plt::Tn3-nice fusion, indicating that inaZ was transcribed from a promoter of the plt region. Cells of Pf-5 containing a...Continue Reading

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Citations

Apr 28, 2001·Journal of Experimental Botany·J M Whipps
Jul 6, 2000·Applied and Environmental Microbiology·C A WhistlerJ E Loper
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Maria Péchy-TarrChristoph Keel
Nov 10, 2001·Annual Review of Phytopathology·CL BenderJ Loper
Jul 31, 2004·Annual Review of Phytopathology·Eric B Nelson
Mar 11, 2005·Nature Reviews. Microbiology·Dieter Haas, Geneviève Défago
Nov 5, 2005·Applied and Environmental Microbiology·Marion BrodhagenJoyce E Loper
Apr 29, 2015·Frontiers in Plant Science·Walaa K Mousa, Manish N Raizada
Feb 24, 2001·Environmental Microbiology·R J EllisM J Bailey
Mar 19, 2008·Microbial Ecology·Popavath Ravindra NaikNatarajan Sakthivel
Feb 9, 2013·Applied Microbiology and Biotechnology·Paramasivan PonrajParamasamy Gunasekaran
Nov 10, 2001·Annual Review of Phytopathology·Kevin P Smith, Robert M Goodman
Apr 21, 2009·Applied and Environmental Microbiology·Patrice de WerraMonika Maurhofer
Mar 27, 1999·Journal of Bacteriology·B Nowak-ThompsonJ E Loper

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