Characterization of a heavy metal ATPase from the apicomplexan Cryptosporidium parvum

Michael J LaGierJ S Keithly


P1-ATPases are transporters which pump heavy metals across membranes, either to provide enzymes with essential cofactors or to remove excess, toxic metal cations from the cytosol. The first protist P1-ATPase (CpATPase2) has been isolated from the apicomplexan Cryptosporidium parvum, an opportunistic pathogen of AIDS patients. This single copy gene encodes 1260 amino acids (aa), predicting a protein of 144.7 kDa. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis confirmed CpATPase2 expression. Immunofluorescence microscopy of C. parvum sporozoites using rabbit antiserum raised against a glutathione-S-transferase (GST) fusion protein suggests that CpATPase2 is associated with the plasma- and cytoplasmic membranes. The protein shares greatest overall sequence similarity to previously characterized copper P1-ATPases. Expression and subsequent biochemical analyses of the N-terminal heavy metal binding domain (HMBD, GMxCxxC) of CpATPase2 as a maltose-binding protein (MBP) in Escherichia coli reveals that the protein specifically binds reduced copper, Cu(I), in vitro and in vivo, and that the cysteine residues of HMBD are responsible for heavy metal coordination. Overall, these data show that the apico...Continue Reading


Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Munro, T Maniatis
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Jan 1, 1985·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·E PiccinniL Guidolin
Dec 1, 1994·Computer Applications in the Biosciences : CABIOS·M G Claros, G von Heijne
Mar 20, 1995·Analytical Biochemistry·A J Brenner, E D Harris
Jun 6, 1994·FEBS Letters·M SoliozR Krapf
Jan 5, 1996·The Journal of Biological Chemistry·K MelchersK P Schäfer
May 6, 1996·Biochimica Et Biophysica Acta·J V MøllerM le Maire
Jun 1, 1996·Molecular and Biochemical Parasitology·M DyerR Mikkelsen
Sep 1, 1997·Molecular and Biochemical Parasitology·J S KeithlyN Yarlett
Jan 23, 1998·Nature Structural Biology·J GitschierW J Fairbrother
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Lutsenko, M J Cooper
Jun 19, 1998·Molecular Microbiology·D D Agranoff, S Krishna
Apr 17, 1999·Cell Structure and Function·M MaedaR Sato
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E PerkinsSylvie M Le Blancq
Dec 11, 1999·Molecular and Biochemical Parasitology·M KimuraH Ogura
Feb 29, 2000·Molecular and Biochemical Parasitology·G ZhuJ S Keithly
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·Z WeissmanD Kornitzer
Apr 1, 2000·Current Opinion in Chemical Biology·M Vasák, D W Hasler
Apr 5, 2000·Biochimica Et Biophysica Acta·L E WilliamsJ L Hall


Jun 14, 2002·International Journal for Parasitology·Michael J LaGierG Zhu
Jan 9, 2013·Clinical Microbiology Reviews·Maha BouzidKevin M Tyler
Feb 4, 2009·Experimental Parasitology·Stanley Dean Rider, G Zhu
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies

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